Baculovirus expression of mouse lactoferrin receptor and tissue distribution in the mouse

Yasushi A. Suzuki, Bo Lönnerdal

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Lactoferrin (Lf) has been shown to have a role in the immune system and in early development of the mouse embryo. A specific receptor for Lf has been suggested to mediate the functions of Lf. We have recently identified a Lf receptor (LfR) in human fetal small intestine. We therefore hypothesized that the mouse homologue of this protein functions as a LfR. We expressed mouse Lf (MLf) and the mouse homologue (MLfR) in a baculovirus-insect cell system. The recombinant MLfR (rMLfR) was purified by immobilized recombinant MLf (rMLf) affinity chromatography, demonstrating an interaction between rMLf and the rMLfR. RT-PCR revealed that MLfR was expressed in various tissues and during embryonic development. Immunohistochemical analysis revealed that the MLfR was localized in various tissues including small intestinal epithelium, stomach, kidney, ovary, and various regions of brain. In summary, the MLfR functions as a receptor for MLf, is expressed and localized in various tissues, and may be involved in the indispensable function of MLf during early embryonic development.

Original languageEnglish (US)
Pages (from-to)301-309
Number of pages9
JournalBioMetals
Volume17
Issue number3
DOIs
StatePublished - Jun 2004

Keywords

  • Cloning
  • Immunohistochemistry
  • Lactoferrin receptor
  • Mouse lactoferrin
  • Recombinant protein

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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