Bacteriological and electron microscopy examination of brown pigment stones

Joseph Leung, J. Y. Sung, J. W. Costerton

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Bacteriological and morphological studies of 38 brown pigment common duct stones were performed. Stone cultures were positive for bacteria in 80.5% of those studied. Enterococci were the most common organisms that were isolated. Scanning electron microscopy showed the presence of bacteria in 84.2% of the stones. The bacteria were seen embedded within an amorphous matrix in alternating layers of flakelike crystals. Transmission electron microscopy showed the presence of gram-positive and gram-negative bacteria surrounded by a ruthenium red-stained exopolysaccharide material. Results of the bacteriological and morphological studies confirmed the close relationship between the presence of bacteria and the development of brown pigment stones.

Original languageEnglish (US)
Pages (from-to)915-921
Number of pages7
JournalJournal of Clinical Microbiology
Volume27
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Electron Microscopy
Bacteria
Ruthenium Red
Enterococcus
Gram-Negative Bacteria
Transmission Electron Microscopy
Electron Scanning Microscopy

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Bacteriological and electron microscopy examination of brown pigment stones. / Leung, Joseph; Sung, J. Y.; Costerton, J. W.

In: Journal of Clinical Microbiology, Vol. 27, No. 5, 1989, p. 915-921.

Research output: Contribution to journalArticle

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