Bactericidal potency and mechanistic specificity of neutrophil defensins against bovine mastitis pathogens

James S Cullor, S. Wood, W. Smith, L. Panico, M. E. Selsted

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Two neutrophil defensins, rabbit peptides NP-1 and NP-5, were examined for their in vitro antibacterial activity against a panel of Gram-positive and Gram-negative organisms isolated from mastitic cows. Incubation for 60 minutes with 5 μg/ml of NP-1 in 10 mM sodium phosphate buffer resulted in substantial killing (> 95%) of all organisms tested. Although NP-5 was virtually inactive under these same conditions, supplementation of the incubation mixture with dilute nutrient media sensitized otherwise resistant organisms to this defensin. For both NP-1 and NP-5, bactericidal activity was dependent on time and concentration. Our findings demonstrate that the spectrum of defensin bactericidal activities include clinically important bovine pathogens. Further, the data demonstrate that NP-1 and NP-5, peptides which are homologous in 18 of their 33 residue positions, possess distinct mechanisms of action.

Original languageEnglish (US)
Pages (from-to)49-58
Number of pages10
JournalVeterinary Microbiology
Volume29
Issue number1
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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