Bacterial invasion of the biliary system by way of the portal-venous system

Joseph Y. Sung, Eldon A. Shaffer, Merle E. Olson, Joseph Leung, Kan Lam, J. William Costerton

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

It has been suggested that bacteria in the intestine gain access into the biliary tract by entering the portal-venous blood. We have tested the hypothesis of hematogenous infection of the biliary system in cats. The animals were treated in three different groups: group A (no biliary obstruction), group B (acute biliary obstruction) and group C (chronic biliary obstruction). A mutant strain of Escherichia coli was infused into the splenic vein of cats at three different dosages (107, 105 and 103) with sham controls. In the unobstructed biliary system, the mutant E. coli was isolated from the bile 30 min and 90 min after the infusion of 107 and 106 E. coli, respectively. No bacteria were found in the bile with the infusion of 103 E. coli and in the control animals. Bile flow was significantly reduced with the infusion of bacteria. The biliary excretion of E. coli in group B was similar to that in group A. In group C, the bile output in the first hour was very high but declined rapidly. E. coli was excreted into the bile at all three dosages of infusion after 30 min. Histological sections of the liver showed that the infused bacteria entered the sinusoidal blood and that some were phagocytosed by Kupffer cells. The portal-venous blood was considered an important route of bacterial invasion into the biliary system, and the penetration of bacteria was facilitated in biliary obstruction.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalHepatology
Volume14
Issue number2
StatePublished - Aug 1991
Externally publishedYes

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Portal System
Biliary Tract
Bile
Escherichia coli
Bacteria
Cats
Splenic Vein
Kupffer Cells
Phagocytosis
Intestines
Liver
Infection

ASJC Scopus subject areas

  • Hepatology

Cite this

Sung, J. Y., Shaffer, E. A., Olson, M. E., Leung, J., Lam, K., & Costerton, J. W. (1991). Bacterial invasion of the biliary system by way of the portal-venous system. Hepatology, 14(2), 313-317.

Bacterial invasion of the biliary system by way of the portal-venous system. / Sung, Joseph Y.; Shaffer, Eldon A.; Olson, Merle E.; Leung, Joseph; Lam, Kan; Costerton, J. William.

In: Hepatology, Vol. 14, No. 2, 08.1991, p. 313-317.

Research output: Contribution to journalArticle

Sung, JY, Shaffer, EA, Olson, ME, Leung, J, Lam, K & Costerton, JW 1991, 'Bacterial invasion of the biliary system by way of the portal-venous system', Hepatology, vol. 14, no. 2, pp. 313-317.
Sung JY, Shaffer EA, Olson ME, Leung J, Lam K, Costerton JW. Bacterial invasion of the biliary system by way of the portal-venous system. Hepatology. 1991 Aug;14(2):313-317.
Sung, Joseph Y. ; Shaffer, Eldon A. ; Olson, Merle E. ; Leung, Joseph ; Lam, Kan ; Costerton, J. William. / Bacterial invasion of the biliary system by way of the portal-venous system. In: Hepatology. 1991 ; Vol. 14, No. 2. pp. 313-317.
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