Abstract
Amphibians are experiencing global declines due in part to the infectious disease chytridiomycosis. Some symbiotic bacteria residents on frog skin have been shown to inhibit the growth of Batrachochytrium dendrobatitis (Bd) but few studies have attempted to fully describe the resident bacterial flora of frog skin. We cultured and sequenced 130 bacterial isolates from frogs collected from the California Klamath Range, recovering predominantly Gram-negative bacteria from 20 higher order taxa and 31 genera. There were also a large number of unclassifiable isolates. Forty-three isolates were assessed for their ability to inhibit the growth of Bd in vitro; of these, two had strong and three had slight anti-Bd activity. We suggest that many bacterial species may play a secondary role in Bd resistance, acting synergistically with inhibitory species. Future research is required in order to characterize these interactions. Understanding the relationships between bacterial strains may be important in predicting and managing the effects of future anti-Bd treatments such as antimicrobial compounds or probiotic bacteria.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 591-598 |
| Number of pages | 8 |
| Journal | Comparative Immunology, Microbiology and Infectious Diseases |
| Volume | 36 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2013 |
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Keywords
- Bacteria
- Batrachochytrium dendrobatidis
- Biofilm
- Chytrid
- Frog
- Innate immunity
ASJC Scopus subject areas
- Immunology
- Microbiology
- Infectious Diseases
- Immunology and Allergy
- veterinary(all)
Cite this
Bacterial flora on Cascades frogs in the Klamath mountains of California. / Roth, Tara; Foley, Janet E; Worth, Joy; Piovia-Scott, Jonah; Pope, Karen; Lawler, Sharon.
In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 36, No. 6, 12.2013, p. 591-598.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bacterial flora on Cascades frogs in the Klamath mountains of California
AU - Roth, Tara
AU - Foley, Janet E
AU - Worth, Joy
AU - Piovia-Scott, Jonah
AU - Pope, Karen
AU - Lawler, Sharon
PY - 2013/12
Y1 - 2013/12
N2 - Amphibians are experiencing global declines due in part to the infectious disease chytridiomycosis. Some symbiotic bacteria residents on frog skin have been shown to inhibit the growth of Batrachochytrium dendrobatitis (Bd) but few studies have attempted to fully describe the resident bacterial flora of frog skin. We cultured and sequenced 130 bacterial isolates from frogs collected from the California Klamath Range, recovering predominantly Gram-negative bacteria from 20 higher order taxa and 31 genera. There were also a large number of unclassifiable isolates. Forty-three isolates were assessed for their ability to inhibit the growth of Bd in vitro; of these, two had strong and three had slight anti-Bd activity. We suggest that many bacterial species may play a secondary role in Bd resistance, acting synergistically with inhibitory species. Future research is required in order to characterize these interactions. Understanding the relationships between bacterial strains may be important in predicting and managing the effects of future anti-Bd treatments such as antimicrobial compounds or probiotic bacteria.
AB - Amphibians are experiencing global declines due in part to the infectious disease chytridiomycosis. Some symbiotic bacteria residents on frog skin have been shown to inhibit the growth of Batrachochytrium dendrobatitis (Bd) but few studies have attempted to fully describe the resident bacterial flora of frog skin. We cultured and sequenced 130 bacterial isolates from frogs collected from the California Klamath Range, recovering predominantly Gram-negative bacteria from 20 higher order taxa and 31 genera. There were also a large number of unclassifiable isolates. Forty-three isolates were assessed for their ability to inhibit the growth of Bd in vitro; of these, two had strong and three had slight anti-Bd activity. We suggest that many bacterial species may play a secondary role in Bd resistance, acting synergistically with inhibitory species. Future research is required in order to characterize these interactions. Understanding the relationships between bacterial strains may be important in predicting and managing the effects of future anti-Bd treatments such as antimicrobial compounds or probiotic bacteria.
KW - Bacteria
KW - Batrachochytrium dendrobatidis
KW - Biofilm
KW - Chytrid
KW - Frog
KW - Innate immunity
UR - http://www.scopus.com/inward/record.url?scp=84886384855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886384855&partnerID=8YFLogxK
U2 - 10.1016/j.cimid.2013.07.002
DO - 10.1016/j.cimid.2013.07.002
M3 - Article
C2 - 23969031
AN - SCOPUS:84886384855
VL - 36
SP - 591
EP - 598
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
SN - 0147-9571
IS - 6
ER -