Bacterial distribution and tissue targets following experimental Edwardsiella ictaluri infection in Nile tilapia Oreochromis niloticus

Esteban Soto Martinez, Oscar Illanes, Floyd Revan, Matt Griffin, Andrés Riofrio

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Edwardsiella ictaluri, a Gram-negative enteric bacterium, is the known etiological agent of enteric septicemia of catfish. In the last few years, different strains have been implicated as the causative agent of mortality events in cultured fish, including Nile tilapia Oreochromis niloticus L. Due to the emergent nature of edwardsiellosis in non-ictalurid fish, little is known about the dynamics of E. ictaluri infection in tilapia. The purpose of this study was to gain a better understanding of the pathogenesis of edwardsiellosis in tilapia by determining the median lethal and infective doses, tissue targets of infection, rate of bacterial dissemination, and the specific tissue response to E. ictaluri following an immersion challenge with bacterial strains recovered from outbreak events in tilapia. In addition to histopathology assessment, the bacterial burdens in several tissues of infected fish were determined over a 2 wk course of infection using quantitative real-time PCR (qPCR). The collected data suggest the cutaneous and oral routes as the main ports of entry for the organism, which later spreads hematogenously throughout the body. Even though histopathological assessment of infected fish revealed involvement of a wide range of tissues, the severity of the necrotizing and granulomatous lesions in the spleen and head kidney, with concomitant high levels of bacterial DNA in these organs determined by qPCR, identifies them as the main targets of infection.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalDiseases of Aquatic Organisms
Volume104
Issue number2
DOIs
StatePublished - May 27 2013
Externally publishedYes

Fingerprint

Edwardsiella ictaluri
Oreochromis niloticus
Tilapia (Cichlidae)
fish
infection
histopathology
etiological agents
tilapia (common name)
lethal genes
intestinal microorganisms
lesion
lesions (animal)
mouth
spleen
quantitative polymerase chain reaction
pathogenesis
kidneys
DNA
mortality
bacterium

Keywords

  • Edwardsiella ictaluri
  • Histopathology
  • Pathogenesis
  • Real-time PCR
  • Tilapia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Bacterial distribution and tissue targets following experimental Edwardsiella ictaluri infection in Nile tilapia Oreochromis niloticus. / Soto Martinez, Esteban; Illanes, Oscar; Revan, Floyd; Griffin, Matt; Riofrio, Andrés.

In: Diseases of Aquatic Organisms, Vol. 104, No. 2, 27.05.2013, p. 105-112.

Research output: Contribution to journalArticle

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