(-)-Baclofen and γ-aminobutyric acid inhibit calcium currents in isolated retinal ganglion cells

V. P. Bindokas, Andrew Ishida

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Of the various synaptic inputs known to converge upon retinal ganglion cells, the major inhibitory inputs are thought to be GABAergic. Although γ-aminobutyric add (GABA) is known to activate anion-selective ion channels in retinal ganglion cells, we have tested the possibility that GABA can also modulate cationic conductances in these cells, as seen in other central and peripheral neurons. Specifically, we have made whole-cell patch-clamp recordings to test whether voltage-gated calcium currents in isolated goldfish retinal ganglion cells are sensitive to GABAB receptor ligands. (-)-Baclofen and GABA inhibited calcium currents activated by moderately long depolarizations and, during large depolarisations (e.g., to 0 mV), also appeared to accelerate the rate of current decay. The calcium current inhibition induced by (-)-baclofen and GABA was not prevented by 2-hydroxyasclofen, phaclofen, or bicuculline, even though bicuculline suppressed a GABA-activated conductance in these cells. These results demonstrate the presence of baclofen- and GABA-sentive calcium currents in vertebrate retinal ganglion cells as well as the coexistence of GABAA and GABAB receptors in individual retinal ganglion cells.

Original languageEnglish (US)
Pages (from-to)10759-10763
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number23
StatePublished - 1991

ASJC Scopus subject areas

  • General
  • Genetics

Fingerprint Dive into the research topics of '(-)-Baclofen and γ-aminobutyric acid inhibit calcium currents in isolated retinal ganglion cells'. Together they form a unique fingerprint.

  • Cite this