Bacillary haemoglobinuria diagnosis by the peroxidase-antiperoxidase (PAP) technique.

Francisco A Uzal, K. Belak, E. Rivera, C. A. Robles, R. E. Feinstein

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


A peroxidase-antiperoxidase (PAP) technique was used to diagnose bacillary haemoglobinuria in formalin-fixed, paraffin-embedded liver tissues of cattle. The PAP method revealed Clostridium haemolyticum in the zone of liver necrosis characteristic of the disease and also in culture smears of this microorganism, but C. novyi type B, C. chauvoei, C. septicum and C. perfringens types B and C remained unstained by the PAP reaction. The PAP technique performed provides a specific, simple and rapid method to diagnose bacillary haemoglobinuria.

Original languageEnglish (US)
Pages (from-to)595-598
Number of pages4
JournalZentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B
Issue number8
StatePublished - Oct 1992
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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