B94, a primary response gene inducible by tumor necrosis factor-α, is expressed in developing hematopoietic tissues and the sperm acrosome

Frederick W. Wolf, Vidya Sarma, Michael F Seldin, Sandra Drake, Suzanne J. Suchard, Haining Shao, K. Sue O'Shea, Vishva M. Dixit

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

B94 was originally described as a novel tumor necrosis factor-α-inducible primary response gene in endothelial cells which was also induced in an in vitro model of angiogenesis. To further characterize its expression, we cloned the mouse homologue and mapped its developmental and tissue specific expression. The predicted amino acid sequence of mouse B94 was found to be 83% similar to its human homologue. The gene was localized to mouse chromosome 12 just centromeric to the immunoglobulin heavy chain locus, in a region that is often rearranged in T-cell neoplasms. To explore the possibility that B94 is expressed during vasculogenesis and other developmental processes, the expression of its transcript was determined during mouse development by in situ hybridization. In 10-day embryos B94 was expressed prominently in the myocardium and in the aortic arch. By the 15th day of gestation, expression was restricted largely to the liver, the bone forming regions of the jaw, the aortic endothelium, and the nasopharynx: a pattern that was maintained until just prior to birth. Postnatally, expression shifted to the red pulp of the spleen and the thymic medulla. B94 expression was extinguished in most adult tissues but was detectable in lymphopoietic tissues including the spleen, tonsil, and lymphatic aggregates in the gut. Consistent with this was the finding that mononuclear progenitor cells in bone marrow and mature peripheral blood monocytes expressed B94. A truncated testis-specific transcript previously identified by Northern blot analysis was determined to result from the use of an alternate polyadenylation signal which was surprisingly located within the open reading frame. This shorter transcript was expressed at high levels exclusively in late stage spermatids. Immunostaining with an affinity-purified polyclonal antiserum revealed B94 to be localized to the acrosomal compartment of mature sperm. These studies demonstrate that B94 expression is tightly regulated during development and suggests distinct roles for B94 in myelopoiesis and spermatogenesis.

Original languageEnglish (US)
Pages (from-to)3633-3640
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number5
StatePublished - Feb 4 1994
Externally publishedYes

Fingerprint

Acrosome
Spermatozoa
Tumor Necrosis Factor-alpha
Genes
Tissue
Bone
Immunoglobulin Heavy Chains
T-cells
Spleen
Endothelial cells
Arches
Myelopoiesis
Chromosomes
Chromosomes, Human, Pair 12
Liver
Polyadenylation
Pulp
Spermatids
Nasopharynx
Immune Sera

ASJC Scopus subject areas

  • Biochemistry

Cite this

B94, a primary response gene inducible by tumor necrosis factor-α, is expressed in developing hematopoietic tissues and the sperm acrosome. / Wolf, Frederick W.; Sarma, Vidya; Seldin, Michael F; Drake, Sandra; Suchard, Suzanne J.; Shao, Haining; O'Shea, K. Sue; Dixit, Vishva M.

In: Journal of Biological Chemistry, Vol. 269, No. 5, 04.02.1994, p. 3633-3640.

Research output: Contribution to journalArticle

Wolf, Frederick W. ; Sarma, Vidya ; Seldin, Michael F ; Drake, Sandra ; Suchard, Suzanne J. ; Shao, Haining ; O'Shea, K. Sue ; Dixit, Vishva M. / B94, a primary response gene inducible by tumor necrosis factor-α, is expressed in developing hematopoietic tissues and the sperm acrosome. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 5. pp. 3633-3640.
@article{5b573c2406e24208b40c1235cdb793f4,
title = "B94, a primary response gene inducible by tumor necrosis factor-α, is expressed in developing hematopoietic tissues and the sperm acrosome",
abstract = "B94 was originally described as a novel tumor necrosis factor-α-inducible primary response gene in endothelial cells which was also induced in an in vitro model of angiogenesis. To further characterize its expression, we cloned the mouse homologue and mapped its developmental and tissue specific expression. The predicted amino acid sequence of mouse B94 was found to be 83{\%} similar to its human homologue. The gene was localized to mouse chromosome 12 just centromeric to the immunoglobulin heavy chain locus, in a region that is often rearranged in T-cell neoplasms. To explore the possibility that B94 is expressed during vasculogenesis and other developmental processes, the expression of its transcript was determined during mouse development by in situ hybridization. In 10-day embryos B94 was expressed prominently in the myocardium and in the aortic arch. By the 15th day of gestation, expression was restricted largely to the liver, the bone forming regions of the jaw, the aortic endothelium, and the nasopharynx: a pattern that was maintained until just prior to birth. Postnatally, expression shifted to the red pulp of the spleen and the thymic medulla. B94 expression was extinguished in most adult tissues but was detectable in lymphopoietic tissues including the spleen, tonsil, and lymphatic aggregates in the gut. Consistent with this was the finding that mononuclear progenitor cells in bone marrow and mature peripheral blood monocytes expressed B94. A truncated testis-specific transcript previously identified by Northern blot analysis was determined to result from the use of an alternate polyadenylation signal which was surprisingly located within the open reading frame. This shorter transcript was expressed at high levels exclusively in late stage spermatids. Immunostaining with an affinity-purified polyclonal antiserum revealed B94 to be localized to the acrosomal compartment of mature sperm. These studies demonstrate that B94 expression is tightly regulated during development and suggests distinct roles for B94 in myelopoiesis and spermatogenesis.",
author = "Wolf, {Frederick W.} and Vidya Sarma and Seldin, {Michael F} and Sandra Drake and Suchard, {Suzanne J.} and Haining Shao and O'Shea, {K. Sue} and Dixit, {Vishva M.}",
year = "1994",
month = "2",
day = "4",
language = "English (US)",
volume = "269",
pages = "3633--3640",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

TY - JOUR

T1 - B94, a primary response gene inducible by tumor necrosis factor-α, is expressed in developing hematopoietic tissues and the sperm acrosome

AU - Wolf, Frederick W.

AU - Sarma, Vidya

AU - Seldin, Michael F

AU - Drake, Sandra

AU - Suchard, Suzanne J.

AU - Shao, Haining

AU - O'Shea, K. Sue

AU - Dixit, Vishva M.

PY - 1994/2/4

Y1 - 1994/2/4

N2 - B94 was originally described as a novel tumor necrosis factor-α-inducible primary response gene in endothelial cells which was also induced in an in vitro model of angiogenesis. To further characterize its expression, we cloned the mouse homologue and mapped its developmental and tissue specific expression. The predicted amino acid sequence of mouse B94 was found to be 83% similar to its human homologue. The gene was localized to mouse chromosome 12 just centromeric to the immunoglobulin heavy chain locus, in a region that is often rearranged in T-cell neoplasms. To explore the possibility that B94 is expressed during vasculogenesis and other developmental processes, the expression of its transcript was determined during mouse development by in situ hybridization. In 10-day embryos B94 was expressed prominently in the myocardium and in the aortic arch. By the 15th day of gestation, expression was restricted largely to the liver, the bone forming regions of the jaw, the aortic endothelium, and the nasopharynx: a pattern that was maintained until just prior to birth. Postnatally, expression shifted to the red pulp of the spleen and the thymic medulla. B94 expression was extinguished in most adult tissues but was detectable in lymphopoietic tissues including the spleen, tonsil, and lymphatic aggregates in the gut. Consistent with this was the finding that mononuclear progenitor cells in bone marrow and mature peripheral blood monocytes expressed B94. A truncated testis-specific transcript previously identified by Northern blot analysis was determined to result from the use of an alternate polyadenylation signal which was surprisingly located within the open reading frame. This shorter transcript was expressed at high levels exclusively in late stage spermatids. Immunostaining with an affinity-purified polyclonal antiserum revealed B94 to be localized to the acrosomal compartment of mature sperm. These studies demonstrate that B94 expression is tightly regulated during development and suggests distinct roles for B94 in myelopoiesis and spermatogenesis.

AB - B94 was originally described as a novel tumor necrosis factor-α-inducible primary response gene in endothelial cells which was also induced in an in vitro model of angiogenesis. To further characterize its expression, we cloned the mouse homologue and mapped its developmental and tissue specific expression. The predicted amino acid sequence of mouse B94 was found to be 83% similar to its human homologue. The gene was localized to mouse chromosome 12 just centromeric to the immunoglobulin heavy chain locus, in a region that is often rearranged in T-cell neoplasms. To explore the possibility that B94 is expressed during vasculogenesis and other developmental processes, the expression of its transcript was determined during mouse development by in situ hybridization. In 10-day embryos B94 was expressed prominently in the myocardium and in the aortic arch. By the 15th day of gestation, expression was restricted largely to the liver, the bone forming regions of the jaw, the aortic endothelium, and the nasopharynx: a pattern that was maintained until just prior to birth. Postnatally, expression shifted to the red pulp of the spleen and the thymic medulla. B94 expression was extinguished in most adult tissues but was detectable in lymphopoietic tissues including the spleen, tonsil, and lymphatic aggregates in the gut. Consistent with this was the finding that mononuclear progenitor cells in bone marrow and mature peripheral blood monocytes expressed B94. A truncated testis-specific transcript previously identified by Northern blot analysis was determined to result from the use of an alternate polyadenylation signal which was surprisingly located within the open reading frame. This shorter transcript was expressed at high levels exclusively in late stage spermatids. Immunostaining with an affinity-purified polyclonal antiserum revealed B94 to be localized to the acrosomal compartment of mature sperm. These studies demonstrate that B94 expression is tightly regulated during development and suggests distinct roles for B94 in myelopoiesis and spermatogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0028145703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028145703&partnerID=8YFLogxK

M3 - Article

C2 - 8106408

AN - SCOPUS:0028145703

VL - 269

SP - 3633

EP - 3640

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 5

ER -