Abstract
We assessed the role of B7-1 and B7-2 costimulatory molecules on the course of murine Lyme borreliosis because experimental Lyme arthritis is dependent, at least partially, upon the development of the host immune response and these costimulatory molecules have been implicated in CD4+ T- cell differentiation. We demonstrated that Borrelia burgdorferi infection upregulated the surface expression of B7-1 and B7-2 in macrophages and B7-2 expression in B cells. Anti-B7-2 monoclonal antibody (MAb) or both anti-B7-2 and anti-B7-1 MAbs produced a dose-dependent increase in the severity of Lyme arthritis in C3H/HeN mice. In contrast, the administration of an anti-B7-1 MAb reduced the degree of arthritis. These effects occurred independently of significant alteration in B. burgdorferi-specific immune responses, including splenocyte proliferative responses to B. burgdorferi, B. burgdorferi antibody levels and specificity, and mRNA levels of gamma interferon, interleukin-4 (IL-4), IL-10, and IL-12 in the spleen. These results demonstrate that signaling delivered by B7-1 and B7-2 plays a role in determining the severity of acute murine Lyme arthritis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3037-3041 |
| Number of pages | 5 |
| Journal | Infection and Immunity |
| Volume | 65 |
| Issue number | 8 |
| State | Published - 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
B7-1 and B7-2 monoclonal antibodies modulate the severity of murine Lyme arthritis. / Anguita, Juan; Roth, Rita; Samanta, Swapna; Gee, Renelle J.; Barthold, Stephen W; Mamula, Mark; Fikrig, Erol.
In: Infection and Immunity, Vol. 65, No. 8, 1997, p. 3037-3041.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - B7-1 and B7-2 monoclonal antibodies modulate the severity of murine Lyme arthritis
AU - Anguita, Juan
AU - Roth, Rita
AU - Samanta, Swapna
AU - Gee, Renelle J.
AU - Barthold, Stephen W
AU - Mamula, Mark
AU - Fikrig, Erol
PY - 1997
Y1 - 1997
N2 - We assessed the role of B7-1 and B7-2 costimulatory molecules on the course of murine Lyme borreliosis because experimental Lyme arthritis is dependent, at least partially, upon the development of the host immune response and these costimulatory molecules have been implicated in CD4+ T- cell differentiation. We demonstrated that Borrelia burgdorferi infection upregulated the surface expression of B7-1 and B7-2 in macrophages and B7-2 expression in B cells. Anti-B7-2 monoclonal antibody (MAb) or both anti-B7-2 and anti-B7-1 MAbs produced a dose-dependent increase in the severity of Lyme arthritis in C3H/HeN mice. In contrast, the administration of an anti-B7-1 MAb reduced the degree of arthritis. These effects occurred independently of significant alteration in B. burgdorferi-specific immune responses, including splenocyte proliferative responses to B. burgdorferi, B. burgdorferi antibody levels and specificity, and mRNA levels of gamma interferon, interleukin-4 (IL-4), IL-10, and IL-12 in the spleen. These results demonstrate that signaling delivered by B7-1 and B7-2 plays a role in determining the severity of acute murine Lyme arthritis.
AB - We assessed the role of B7-1 and B7-2 costimulatory molecules on the course of murine Lyme borreliosis because experimental Lyme arthritis is dependent, at least partially, upon the development of the host immune response and these costimulatory molecules have been implicated in CD4+ T- cell differentiation. We demonstrated that Borrelia burgdorferi infection upregulated the surface expression of B7-1 and B7-2 in macrophages and B7-2 expression in B cells. Anti-B7-2 monoclonal antibody (MAb) or both anti-B7-2 and anti-B7-1 MAbs produced a dose-dependent increase in the severity of Lyme arthritis in C3H/HeN mice. In contrast, the administration of an anti-B7-1 MAb reduced the degree of arthritis. These effects occurred independently of significant alteration in B. burgdorferi-specific immune responses, including splenocyte proliferative responses to B. burgdorferi, B. burgdorferi antibody levels and specificity, and mRNA levels of gamma interferon, interleukin-4 (IL-4), IL-10, and IL-12 in the spleen. These results demonstrate that signaling delivered by B7-1 and B7-2 plays a role in determining the severity of acute murine Lyme arthritis.
UR - http://www.scopus.com/inward/record.url?scp=0030822736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030822736&partnerID=8YFLogxK
M3 - Article
C2 - 9234751
AN - SCOPUS:0030822736
VL - 65
SP - 3037
EP - 3041
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 8
ER -