B7-1 and B7-2 monoclonal antibodies modulate the severity of murine Lyme arthritis

Juan Anguita, Rita Roth, Swapna Samanta, Renelle J. Gee, Stephen W Barthold, Mark Mamula, Erol Fikrig

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We assessed the role of B7-1 and B7-2 costimulatory molecules on the course of murine Lyme borreliosis because experimental Lyme arthritis is dependent, at least partially, upon the development of the host immune response and these costimulatory molecules have been implicated in CD4+ T- cell differentiation. We demonstrated that Borrelia burgdorferi infection upregulated the surface expression of B7-1 and B7-2 in macrophages and B7-2 expression in B cells. Anti-B7-2 monoclonal antibody (MAb) or both anti-B7-2 and anti-B7-1 MAbs produced a dose-dependent increase in the severity of Lyme arthritis in C3H/HeN mice. In contrast, the administration of an anti-B7-1 MAb reduced the degree of arthritis. These effects occurred independently of significant alteration in B. burgdorferi-specific immune responses, including splenocyte proliferative responses to B. burgdorferi, B. burgdorferi antibody levels and specificity, and mRNA levels of gamma interferon, interleukin-4 (IL-4), IL-10, and IL-12 in the spleen. These results demonstrate that signaling delivered by B7-1 and B7-2 plays a role in determining the severity of acute murine Lyme arthritis.

Original languageEnglish (US)
Pages (from-to)3037-3041
Number of pages5
JournalInfection and Immunity
Volume65
Issue number8
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Anguita, J., Roth, R., Samanta, S., Gee, R. J., Barthold, S. W., Mamula, M., & Fikrig, E. (1997). B7-1 and B7-2 monoclonal antibodies modulate the severity of murine Lyme arthritis. Infection and Immunity, 65(8), 3037-3041.