B lymphocyte lineage cells in newborn and very young NZB mice

Evidence for regulatory disorders affecting B cell formation

H. Jyonouchi, P. W. Kincade, R. A. Good, M. Eric Gershwin

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Abstract

As adults, NZB mice have a severe deficiency of identifiable precursors of B lymphocytes, but this is preceded by a time when B cells and their immediate precursors are present in bone marrow in elevated numbers. Our present studies indicate that the final stages of B cell formation may be proceeding at a greater than normal rate at 4 wk of age. At this time, large numbers of slg- B cell precursors can be identified in NZB but not in CBA/H or DBA/2 marrow that can respond to mitogens in semisolid agar cultures without preculture. Colony formation by these precursors was dependent on the presence of Sephadex G-10-adherent cells in the suspensions. In this respect, 4-wk-old NZB marrow was similar to spleen and liver of normal CBA/H newborn mice. Mixing experiments suggested that hyperactive regulatory cells present in young NZB marrow may promote functional maturation of normal pre-B cells from CBA/H or DBA/2 mice. In addition, potent substances present in the serum of young but not older NZB mice produced the same effect. Factor-mediated enhancement of clonal proliferation by slg- precursors was not dependent on adherent cells and required more than brief exposure of the cells to young NZB serum. These are all indications that abnormalities of B lineage differentiation in young NZB marrow may be attributable to microenvironmental elements. Congeneic, B cell-deficient NZB.xid mice, however, lacked hyperactive regulatory cell function and serum factors that characterize NZB mice of this age. A study was also made of B cell precursors in newborn spleen and liver of NZB mice, and abnormal shifts of populations of B lineage cells were already present by that time. These observations support previous contentions that humoral immunity is precocious and abnormally regulated in NZB mice.

Original languageEnglish (US)
Pages (from-to)2219-2225
Number of pages7
JournalJournal of Immunology
Volume131
Issue number5
StatePublished - 1983
Externally publishedYes

Fingerprint

Inbred NZB Mouse
B-Lymphocytes
B-Lymphoid Precursor Cells
Bone Marrow
Spleen
Serum
Inbred DBA Mouse
Liver
Humoral Immunity
Mitogens
Agar
Suspensions
Population

ASJC Scopus subject areas

  • Immunology

Cite this

B lymphocyte lineage cells in newborn and very young NZB mice : Evidence for regulatory disorders affecting B cell formation. / Jyonouchi, H.; Kincade, P. W.; Good, R. A.; Gershwin, M. Eric.

In: Journal of Immunology, Vol. 131, No. 5, 1983, p. 2219-2225.

Research output: Contribution to journalArticle

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