B-cell lymphoma with Mott cell differentiation in two young adult dogs

Nicole I. Stacy, Mary B. Nabity, Nicole Hackendahl, Melanie Buote, Jennifer Ward, Pamela E. Ginn, William Vernau, William L. Clapp, John W. Harvey

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Two young adult dogs with gastrointestinal signs were each found to have an intra-abdominal mass based on physical examination and diagnostic imaging. On exploratory laparotomy, small intestinal masses and mesenteric lymphadenopathy were found in both dogs; a liver mass was also found in dog 1. Cytologic and histologic examination of intestinal and liver masses and mesenteric lymph nodes revealed 2 distinct lymphoid cell populations: lymphoblasts and atypical Mott cells. With Romanowsky stains, the atypical Mott cells contained many discrete, clear to pale blue cytoplasmic inclusions consistent with Russell bodies that were positive by immunohistochemistry for IgM and CD79a in both dogs and for IgG in dog 2. The Mott cells and occasional lymphoblasts stained strongly positive with periodic acid-Schiff. Using flow cytometric immunophenotyping in dog 1, 60% of peripheral blood mononuclear cells and 85% of cells in an affected lymph node were positive for CD21, CD79a, IgM, and MCH II, indicative of B-cells. With electron microscopy, disorganized and dilated endoplasmic reticulum was seen in Mott cells in tumors from both dogs. Antigen receptor gene rearrangement analysis of lymph node and intestinal masses indicated a clonal B-cell population. Based on cell morphology, tissue involvement, and evidence for clonal B-cell proliferation, we diagnosed neoplasms involving Mott cells. To the authors' knowledge, this is the second report of Mott cell tumors or, more appropriately, B-cell lymphoma with Mott cell differentiation, in dogs. More complete characterization of this neoplasm requires further investigation of additional cases. This lymphoproliferative disease should be considered as a differential diagnosis for canine gastrointestinal tumors.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalVeterinary Clinical Pathology
Volume38
Issue number1
DOIs
StatePublished - Mar 2009

Keywords

  • B-cell
  • Canine
  • Intestinal
  • Lymphoma
  • Mott cells
  • Russell bodies

ASJC Scopus subject areas

  • veterinary(all)

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    Stacy, N. I., Nabity, M. B., Hackendahl, N., Buote, M., Ward, J., Ginn, P. E., Vernau, W., Clapp, W. L., & Harvey, J. W. (2009). B-cell lymphoma with Mott cell differentiation in two young adult dogs. Veterinary Clinical Pathology, 38(1), 113-120. https://doi.org/10.1111/j.1939-165X.2008.00101.x