B-cell fate decisions following influenza virus infection

Kristina Rothaeusler, Nicole Baumgarth

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Rapidly induced, specific Ab generated in extrafollicular foci are important components of early immune protection to influenza virus. The signal(s) that prompt B cells to participate in extrafollicular rather than germinal center responses are incompletely understood. To study the regulation of early B-cell differentiation events following influenza infection, we exploited earlier findings of a strong contribution of C12 idiotype-expressing B cells to the primary HA-specific response against influenza A/PR/8/34. Using an idiotype-specific mAb to C12 and labeled HA, in conjunction with multicolor flow cytometry, we followed the fate of C12Id-expressing influenza HA-specific B cells in WT BALB/c mice, requiring neither genetic manipulation nor adoptive cell transfer. Our studies demonstrate that HA-specific C12Id+ B cells are phenotypically indistinguishable from follicular B cells. While they induced both extrafollicular and germinal center responses, extrafollicular responseswere strongly predominant. Provision of increased HA-specific T-cell help increased the magnitude of the extrafollicular response, but did not shift the C12Id+ response toward germinal center formation. Collectively the data are consistent with the hypothesis that B-cell fate determination following activation is a stochastic process in which infection-induced innate signals might drive the preferential expansion of the early extrafollicular response.

Original languageEnglish (US)
Pages (from-to)366-377
Number of pages12
JournalEuropean Journal of Immunology
Volume40
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

Virus Diseases
Orthomyxoviridae
B-Lymphocytes
Germinal Center
Human Influenza
Stochastic Processes
Adoptive Transfer
Infection
Cell Differentiation
Flow Cytometry
T-Lymphocytes

Keywords

  • B cells
  • Cell differentiation
  • Immune regulation
  • Immune responses

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

B-cell fate decisions following influenza virus infection. / Rothaeusler, Kristina; Baumgarth, Nicole.

In: European Journal of Immunology, Vol. 40, No. 2, 02.2010, p. 366-377.

Research output: Contribution to journalArticle

@article{7d80a11a05f249feb8db5a910d1d200a,
title = "B-cell fate decisions following influenza virus infection",
abstract = "Rapidly induced, specific Ab generated in extrafollicular foci are important components of early immune protection to influenza virus. The signal(s) that prompt B cells to participate in extrafollicular rather than germinal center responses are incompletely understood. To study the regulation of early B-cell differentiation events following influenza infection, we exploited earlier findings of a strong contribution of C12 idiotype-expressing B cells to the primary HA-specific response against influenza A/PR/8/34. Using an idiotype-specific mAb to C12 and labeled HA, in conjunction with multicolor flow cytometry, we followed the fate of C12Id-expressing influenza HA-specific B cells in WT BALB/c mice, requiring neither genetic manipulation nor adoptive cell transfer. Our studies demonstrate that HA-specific C12Id+ B cells are phenotypically indistinguishable from follicular B cells. While they induced both extrafollicular and germinal center responses, extrafollicular responseswere strongly predominant. Provision of increased HA-specific T-cell help increased the magnitude of the extrafollicular response, but did not shift the C12Id+ response toward germinal center formation. Collectively the data are consistent with the hypothesis that B-cell fate determination following activation is a stochastic process in which infection-induced innate signals might drive the preferential expansion of the early extrafollicular response.",
keywords = "B cells, Cell differentiation, Immune regulation, Immune responses",
author = "Kristina Rothaeusler and Nicole Baumgarth",
year = "2010",
month = "2",
doi = "10.1002/eji.200939798",
language = "English (US)",
volume = "40",
pages = "366--377",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "2",

}

TY - JOUR

T1 - B-cell fate decisions following influenza virus infection

AU - Rothaeusler, Kristina

AU - Baumgarth, Nicole

PY - 2010/2

Y1 - 2010/2

N2 - Rapidly induced, specific Ab generated in extrafollicular foci are important components of early immune protection to influenza virus. The signal(s) that prompt B cells to participate in extrafollicular rather than germinal center responses are incompletely understood. To study the regulation of early B-cell differentiation events following influenza infection, we exploited earlier findings of a strong contribution of C12 idiotype-expressing B cells to the primary HA-specific response against influenza A/PR/8/34. Using an idiotype-specific mAb to C12 and labeled HA, in conjunction with multicolor flow cytometry, we followed the fate of C12Id-expressing influenza HA-specific B cells in WT BALB/c mice, requiring neither genetic manipulation nor adoptive cell transfer. Our studies demonstrate that HA-specific C12Id+ B cells are phenotypically indistinguishable from follicular B cells. While they induced both extrafollicular and germinal center responses, extrafollicular responseswere strongly predominant. Provision of increased HA-specific T-cell help increased the magnitude of the extrafollicular response, but did not shift the C12Id+ response toward germinal center formation. Collectively the data are consistent with the hypothesis that B-cell fate determination following activation is a stochastic process in which infection-induced innate signals might drive the preferential expansion of the early extrafollicular response.

AB - Rapidly induced, specific Ab generated in extrafollicular foci are important components of early immune protection to influenza virus. The signal(s) that prompt B cells to participate in extrafollicular rather than germinal center responses are incompletely understood. To study the regulation of early B-cell differentiation events following influenza infection, we exploited earlier findings of a strong contribution of C12 idiotype-expressing B cells to the primary HA-specific response against influenza A/PR/8/34. Using an idiotype-specific mAb to C12 and labeled HA, in conjunction with multicolor flow cytometry, we followed the fate of C12Id-expressing influenza HA-specific B cells in WT BALB/c mice, requiring neither genetic manipulation nor adoptive cell transfer. Our studies demonstrate that HA-specific C12Id+ B cells are phenotypically indistinguishable from follicular B cells. While they induced both extrafollicular and germinal center responses, extrafollicular responseswere strongly predominant. Provision of increased HA-specific T-cell help increased the magnitude of the extrafollicular response, but did not shift the C12Id+ response toward germinal center formation. Collectively the data are consistent with the hypothesis that B-cell fate determination following activation is a stochastic process in which infection-induced innate signals might drive the preferential expansion of the early extrafollicular response.

KW - B cells

KW - Cell differentiation

KW - Immune regulation

KW - Immune responses

UR - http://www.scopus.com/inward/record.url?scp=75149191966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75149191966&partnerID=8YFLogxK

U2 - 10.1002/eji.200939798

DO - 10.1002/eji.200939798

M3 - Article

C2 - 19946883

AN - SCOPUS:75149191966

VL - 40

SP - 366

EP - 377

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -