B and T lymphocytes in the bovine mammary gland: Rosette formation and mitogen response

C. E. Schore, Bennie Osburn, D. E. Jasper, D. E. Tyler

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Characterization of lymphocyte subpopulations in the bovine mammary gland was accomplished using cells obtained from dry secretions. Correlation of cell surface properties with functional capacity was attempted by assaying the ability to form erythrocyte-antibody (EA) rosettes, erythrocyte-antibody-complement (EAC) rosettes, and sheep erythrocyte (E) rosettes and the ability to respond to phytohemagglutinin (PHA), Concanavalin A (Con A), and pokeweed mitogen (PWM) in the lymphocyte stimulation test. Results were compared with those obtained for peripheral blood lymphocytes (PBL) from the same animals. Mammary gland lymphocytes (MGL) formed significantly fewer (p < .01) EA and EAC rosettes, but significantly greater (p < .01) E rosettes compared to PBL. MGL were significantly less responsive (p < .05) to mitogens than were PBL. MGL contained a large proportion of T lymphocytes, which do not respond to T lymphocyte mitogens in culture.

Original languageEnglish (US)
Pages (from-to)561-569
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume2
Issue number6
DOIs
StatePublished - Jan 1 1981

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ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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