B-1a and conventional B cells from autoimmune NZB.H-2bm12 mice exhibit similar functional characteristics in vivo

Dennis M. Klinman, Kevin L. Holmes, Jacqueline Conover, Bor Luen Chiang, M. Eric Gershwin

Research output: Contribution to journalArticle

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Abstract

NZB.H-2bm12 mice develop an autoimmune syndrome characterized by the overproduction of anti-DNA antibodies and the expansion of B-1 B cells. Thus, these animals provide a useful model to examine the antigenic specificity, cross-reactivity and functional capability of B-1 versus conventional lymphocytes. Neither the repertoire expressed by in vivo activated Ly-1+ splenic lymphocytes, nor their cross-reactivity, differed significantly from that of conventional splenic B cells. When Ly-1+ cells were cultured in vitro in the presence of lipopolysaccharide plus interleukin-4 or interferon γ, they underwent isotype switching at the same frequency as conventional B cells. Of interest, B-1 cells from the peritoneal cavity were significantly less likely to undergo isotype switching than those from the spleen. These findings indicate that in vivo activated B-1a and conventional B cells from mice with lupus manifest similar functional characteristics.

Original languageEnglish (US)
Pages (from-to)1866-1871
Number of pages6
JournalEuropean Journal of Immunology
Volume23
Issue number8
StatePublished - Aug 1993

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Keywords

  • Autoimmunity
  • B-1 cells
  • Immunoglobulin isotype
  • Lupus
  • Ly-1 cells

ASJC Scopus subject areas

  • Immunology

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