B-1 cells in the bone marrow are a significant source of natural IgM

Youn Soo Choi, Jacquelyn A. Dieter, Kristina Rothaeusler, Zheng Luo, Nicole Baumgarth

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Natural IgM antibodies secreted in the absence of antigenic challenge are important contributors to antimicrobial immunity and tissue homeostasis. Early studies identified BM and, to a lesser extent the spleen, as main tissue sources of this spontaneously secreted IgM. However, the responsible B-cell subset has never been identified. Using multicolor flow cytometry, cell sorting and chimeric mice in which B-1 and B-2 cells and their secreted antibodies are distinguished by their Ig-allotype, we unequivocally identify the natural IgM-secreting cells in spleen and, for the first time, in the BM as IgM + IgD lo/-CD19 hi CD43 + CD5 +/- B-1 cells. The newly identified population of BM B-1 cells shows many of the phenotypic characteristics of splenic B-1 cells but is distinct from B-1 cells in the peritoneal cavity, which generate at best very small amounts of IgM. Antibody-secreting spleen and BM B-1 cells are distinct also from terminally differentiated plasma cells generated from antigen-induced conventional B cells, as they express high levels of surface IgM and CD19 and lack expression of CD138. Overall, these data identify populations of non-terminally differentiated B-1 cells in spleen and BM as the most significant producers of natural IgM.

Original languageEnglish (US)
Pages (from-to)120-129
Number of pages10
JournalEuropean Journal of Immunology
Volume42
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Animal model
  • Antibodies
  • B cells
  • BM
  • Immunoglobulins

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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