Axotomy increases NADPH-diaphorase staining in rat vagal motor neurons

Manuel F. Gonzalez, Frank R Sharp, Stephen M. Sagar

Research output: Contribution to journalArticle

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Abstract

Left cervical vagotomy increased NADPH-diaphorase (NADPH-d) histochemical staining in neuronal perikarya of the ipsilateral dorsal motor nucleus of the vagus (dmnX) and the rostral part of the nucleus ambiguus (nAmb). This effect appeared by 2 days, was maximal around 10 days, and declined by 30 days after vagotomy. Light and dark stained perikarya occurred in dmn X ipsilateral to the vagotomy which could not be explained on the basis of the biochemical or transmitter content of these neurons. It is unlikely that the increases of NADPH-d activity resulted from changes in cholinergic transmission since vagotomy is known to decrease cholinergic enzyme function. Since vagotomy increased both the glucose metabolic rate and NADPH-d staining of dmnX and nAmb in these experiments, it is more likely that these effects represent regenerative metabolic responses to axotomy.

Original languageEnglish (US)
Pages (from-to)417-427
Number of pages11
JournalBrain Research Bulletin
Volume18
Issue number3
DOIs
StatePublished - 1987
Externally publishedYes

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Keywords

  • NADPH-diaphorase Vagus nerve Dorsal motor nucleus of vagus Nucleus ambiguus Vagotomy 2-Deoxyglucose

ASJC Scopus subject areas

  • Neuroscience(all)

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