Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5)

Marina Scarlato; Theodore Xu; Peter Bannerman; Jacqueline Beesley; Usha R. Reddy; Abdolmohammad Rostami; Steven S. Scherer; David E Pleasure

doi:10.1002/jnr.1193

Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5)

Marina Scarlato, Theodore Xu, Peter Bannerman, Jacqueline Beesley, Usha R. Reddy, Abdolmohammad Rostami, Steven S. Scherer, David E Pleasure

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family. J. Neurosci. Res.

Original language	English (US)
Pages (from-to)	16-22
Number of pages	7
Journal	Journal of Neuroscience Research
Volume	66
Issue number	1
DOIs	https://doi.org/10.1002/jnr.1193
State	Published - Oct 1 2001
Externally published	Yes

Keywords

Axotomy
CAMP
Neuropathy
Wallerian degeneration

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1002/jnr.1193

Cite this

Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5). / Scarlato, Marina; Xu, Theodore; Bannerman, Peter; Beesley, Jacqueline; Reddy, Usha R.; Rostami, Abdolmohammad; Scherer, Steven S.; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 66, No. 1, 01.10.2001, p. 16-22.

Research output: Contribution to journal › Article › peer-review

@article{7a07fedef77e4e87b9f8ebb8a53d58bb,

title = "Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5)",

abstract = "We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family. J. Neurosci. Res.",

keywords = "Axotomy, CAMP, Neuropathy, Wallerian degeneration",

author = "Marina Scarlato and Theodore Xu and Peter Bannerman and Jacqueline Beesley and Reddy, {Usha R.} and Abdolmohammad Rostami and Scherer, {Steven S.} and Pleasure, {David E}",

year = "2001",

month = oct,

day = "1",

doi = "10.1002/jnr.1193",

language = "English (US)",

volume = "66",

pages = "16--22",

journal = "Journal of Neuroscience Research",

issn = "0360-4012",

publisher = "Wiley-Liss Inc.",

number = "1",

}

TY - JOUR

T1 - Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5)

AU - Scarlato, Marina

AU - Xu, Theodore

AU - Bannerman, Peter

AU - Beesley, Jacqueline

AU - Reddy, Usha R.

AU - Rostami, Abdolmohammad

AU - Scherer, Steven S.

AU - Pleasure, David E

PY - 2001/10/1

Y1 - 2001/10/1

N2 - We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family. J. Neurosci. Res.

AB - We screened for genes whose expression is significantly up- or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor-5 (FGF-5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF-5 in axotomized nerve. Axon-Schwann cell interactions decreased the steady-state level of FGF-5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF-5, which is a secreted, neuronotrophic member of the FGF family. J. Neurosci. Res.

KW - Axotomy

KW - CAMP

KW - Neuropathy

KW - Wallerian degeneration

UR - http://www.scopus.com/inward/record.url?scp=0035476273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035476273&partnerID=8YFLogxK

U2 - 10.1002/jnr.1193

DO - 10.1002/jnr.1193

M3 - Article

C2 - 11598998

AN - SCOPUS:0035476273

VL - 66

SP - 16

EP - 22

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 1

ER -

Axon-Schwann cell interactions regulate the expression of fibroblast growth factor-5 (FGF-5)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this