Axolemma is a mitogen for human Schwann cells

G. Sobue, M. J. Brown, S. U. Kim, David E Pleasure

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The mechanisms responsible for the induction of Schwann cell proliferation in peripheral nerves undergoing wallerian degeneration and segmental demyelination are not understood. To determine whether contact with axolemma stimulates mitosis of human Schwann cells, cultured Schwann cells from spinal roots obtained postmortem and from sural nerve biopsy specimens were incubated with axolemmal fractions prepared from human spinal cord or from adult rat central nervous system. Schwann cell proliferation was estimated by autoradiographic assay of tritiated thymidine incorporation. Schwann cell labeling indices after exposure to human or rat axolemmal fractions ranged from 26.7 to 59.9%; labeling indices of Schwann cells cultured without axolemmal fraction were 9.8 to 22.4%. The stimulation index, or ratio of Schwann cell labeling index with axolemmal fraction to that without axolemmal fraction, ranged from 1.97 to 3.40. This study demonstrates that both human and rat axolemma are capable of stimulating human Schwann cell replication in vitro.

Original languageEnglish (US)
Pages (from-to)449-452
Number of pages4
JournalAnnals of Neurology
Volume15
Issue number5
StatePublished - 1984
Externally publishedYes

Fingerprint

Schwann Cells
Mitogens
Cell Proliferation
Wallerian Degeneration
Sural Nerve
Spinal Nerve Roots
Demyelinating Diseases
Peripheral Nerves
Mitosis
Thymidine
Cultured Cells
Spinal Cord
Central Nervous System
Biopsy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sobue, G., Brown, M. J., Kim, S. U., & Pleasure, D. E. (1984). Axolemma is a mitogen for human Schwann cells. Annals of Neurology, 15(5), 449-452.

Axolemma is a mitogen for human Schwann cells. / Sobue, G.; Brown, M. J.; Kim, S. U.; Pleasure, David E.

In: Annals of Neurology, Vol. 15, No. 5, 1984, p. 449-452.

Research output: Contribution to journalArticle

Sobue, G, Brown, MJ, Kim, SU & Pleasure, DE 1984, 'Axolemma is a mitogen for human Schwann cells', Annals of Neurology, vol. 15, no. 5, pp. 449-452.
Sobue, G. ; Brown, M. J. ; Kim, S. U. ; Pleasure, David E. / Axolemma is a mitogen for human Schwann cells. In: Annals of Neurology. 1984 ; Vol. 15, No. 5. pp. 449-452.
@article{808f1407fc724e1faefbe6f3a2c93c59,
title = "Axolemma is a mitogen for human Schwann cells",
abstract = "The mechanisms responsible for the induction of Schwann cell proliferation in peripheral nerves undergoing wallerian degeneration and segmental demyelination are not understood. To determine whether contact with axolemma stimulates mitosis of human Schwann cells, cultured Schwann cells from spinal roots obtained postmortem and from sural nerve biopsy specimens were incubated with axolemmal fractions prepared from human spinal cord or from adult rat central nervous system. Schwann cell proliferation was estimated by autoradiographic assay of tritiated thymidine incorporation. Schwann cell labeling indices after exposure to human or rat axolemmal fractions ranged from 26.7 to 59.9{\%}; labeling indices of Schwann cells cultured without axolemmal fraction were 9.8 to 22.4{\%}. The stimulation index, or ratio of Schwann cell labeling index with axolemmal fraction to that without axolemmal fraction, ranged from 1.97 to 3.40. This study demonstrates that both human and rat axolemma are capable of stimulating human Schwann cell replication in vitro.",
author = "G. Sobue and Brown, {M. J.} and Kim, {S. U.} and Pleasure, {David E}",
year = "1984",
language = "English (US)",
volume = "15",
pages = "449--452",
journal = "Annals of Neurology",
issn = "0364-5134",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Axolemma is a mitogen for human Schwann cells

AU - Sobue, G.

AU - Brown, M. J.

AU - Kim, S. U.

AU - Pleasure, David E

PY - 1984

Y1 - 1984

N2 - The mechanisms responsible for the induction of Schwann cell proliferation in peripheral nerves undergoing wallerian degeneration and segmental demyelination are not understood. To determine whether contact with axolemma stimulates mitosis of human Schwann cells, cultured Schwann cells from spinal roots obtained postmortem and from sural nerve biopsy specimens were incubated with axolemmal fractions prepared from human spinal cord or from adult rat central nervous system. Schwann cell proliferation was estimated by autoradiographic assay of tritiated thymidine incorporation. Schwann cell labeling indices after exposure to human or rat axolemmal fractions ranged from 26.7 to 59.9%; labeling indices of Schwann cells cultured without axolemmal fraction were 9.8 to 22.4%. The stimulation index, or ratio of Schwann cell labeling index with axolemmal fraction to that without axolemmal fraction, ranged from 1.97 to 3.40. This study demonstrates that both human and rat axolemma are capable of stimulating human Schwann cell replication in vitro.

AB - The mechanisms responsible for the induction of Schwann cell proliferation in peripheral nerves undergoing wallerian degeneration and segmental demyelination are not understood. To determine whether contact with axolemma stimulates mitosis of human Schwann cells, cultured Schwann cells from spinal roots obtained postmortem and from sural nerve biopsy specimens were incubated with axolemmal fractions prepared from human spinal cord or from adult rat central nervous system. Schwann cell proliferation was estimated by autoradiographic assay of tritiated thymidine incorporation. Schwann cell labeling indices after exposure to human or rat axolemmal fractions ranged from 26.7 to 59.9%; labeling indices of Schwann cells cultured without axolemmal fraction were 9.8 to 22.4%. The stimulation index, or ratio of Schwann cell labeling index with axolemmal fraction to that without axolemmal fraction, ranged from 1.97 to 3.40. This study demonstrates that both human and rat axolemma are capable of stimulating human Schwann cell replication in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0021369505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021369505&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 449

EP - 452

JO - Annals of Neurology

JF - Annals of Neurology

SN - 0364-5134

IS - 5

ER -