Avian malaria co-infections confound infectivity and vector competence assays of Plasmodium homopolare

Jenny S. Carlson, Brittany Nelms, Chris Barker, William Reisen, Ravinder N.M. Sehgal, Anthony J. Cornel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Currently, there are very few studies of avian malaria that investigate relationships among the host-vector-parasite triad concomitantly. In the current study, we experimentally measured the vector competence of several Culex mosquitoes for a newly described avian malaria parasite, Plasmodium homopolare. Song sparrow (Melospiza melodia) blood infected with a low P. homopolare parasitemia was inoculated into a naïve domestic canary (Serinus canaria forma domestica). Within 5 to 10 days post infection (dpi), the canary unexpectedly developed a simultaneous high parasitemic infection of Plasmodium cathemerium (Pcat6) and a low parasitemic infection of P. homopolare, both of which were detected in blood smears. During this infection period, PCR detected Pcat6, but not P. homopolare in the canary. Between 10 and 60 dpi, Pcat6 blood stages were no longer visible and PCR no longer amplified Pcat6 parasite DNA from canary blood. However, P. homopolare blood stages remained visible, albeit still at very low parasitemias, and PCR was able to amplify P. homopolare DNA. This pattern of mixed Pcat6 and P. homopolare infection was repeated in three secondary infected canaries that were injected with blood from the first infected canary. Mosquitoes that blood-fed on the secondary infected canaries developed infections with Pcat6 as well as another P. cathemerium lineage (Pcat8); none developed PCR detectable P. homopolare infections. These observations suggest that the original P. homopolare-infected songbird also had two un-detectable P. cathemerium lineages/strains. The vector and host infectivity trials in this study demonstrated that current molecular assays may significantly underreport the extent of mixed avian malaria infections in vectors and hosts.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalParasitology Research
DOIs
StateAccepted/In press - May 29 2018

Fingerprint

Avian Malaria
Canaries
avian malaria
vector competence
Plasmodium
Coinfection
mixed infection
Mental Competency
canaries
pathogenicity
Plasmodium cathemerium
assays
Infection
infection
blood
Polymerase Chain Reaction
Parasites
Parasitemia
Culicidae
parasitemia

Keywords

  • Avian malaria
  • Co-infection
  • Culex mosquitoes
  • Experimental infection
  • Plasmodium cathemerium
  • Plasmodium homopolare

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Avian malaria co-infections confound infectivity and vector competence assays of Plasmodium homopolare. / Carlson, Jenny S.; Nelms, Brittany; Barker, Chris; Reisen, William; Sehgal, Ravinder N.M.; Cornel, Anthony J.

In: Parasitology Research, 29.05.2018, p. 1-10.

Research output: Contribution to journalArticle

Carlson, Jenny S. ; Nelms, Brittany ; Barker, Chris ; Reisen, William ; Sehgal, Ravinder N.M. ; Cornel, Anthony J. / Avian malaria co-infections confound infectivity and vector competence assays of Plasmodium homopolare. In: Parasitology Research. 2018 ; pp. 1-10.
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