Autotransplantation in mdx mice of mdx myoblasts genetically corrected by an HSV-1 amplicon vector

Mathieu Bujold, Nicolas Caron, Goeffrey Camiran, Santwana Mukherjee, Paul D. Allen, Jacques P. Tremblay, Yaming Wang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder, characterized by a lack of dystrophin. To eliminate the need for immunosuppressive drugs, transplantation of genetically modified autologous myoblasts has been proposed as a possible therapy for this myopathy. An HSV-1 amplicon vector (HSVDGN), containing a 17.3-kb full-length MCK-driven mouse dystrophin cDNA, an eGFP gene, and a neomycin resistance gene driven by CMV or SV40 promoters, respectively, was constructed and used to transduce mdx primary myoblasts. The presence of the eGFP and neomycin resistance genes facilitated the evaluation of the initial transduction efficiency and the permanent transduction frequency. At low multiplicities of infection (MOI 1-5), the majority of myoblasts (60-90%) expressed GFP. The GFP-positive mdx myoblasts were sorted by FACS and selected with neomycin (300 μg/ml) for 2 weeks. Up to 2% of initially infected mdx myoblasts stably expressed the three transgenes without further selection at that time. These altered cells were grafted into the tibialis anterior muscles of 18 mdx mice. Some of the mice were immunosuppressed with FK506 due to the anticipation that eGFP and the product of neomycin resistance gene might be immunogenic, One month after transplantation, numerous muscle fibers expressing mouse dystrophin were detected by immunohistochemistry, in both immunosuppressed (10-50%) and nonimmunosuppressed (5-25%) mdx mice. Our results demonstrated the capability of permanently expressing a full-length dystrophin in dystrophic myoblasts with HSV-1 amplicon vector and raised the possibility of an eventual treatment of DMD based on the transplantation of genetically modified autologous myoblasts.

Original languageEnglish (US)
Pages (from-to)759-767
Number of pages9
JournalCell Transplantation
Volume11
Issue number8
StatePublished - 2002
Externally publishedYes

Fingerprint

Inbred mdx Mouse
Autologous Transplantation
Myoblasts
Human Herpesvirus 1
Genes
Dystrophin
Neomycin
Muscle
Duchenne Muscular Dystrophy
Transplantation
Muscles
Tacrolimus
Muscular Diseases
Immunosuppressive Agents
Fibers
Transgenes
Complementary DNA
Immunohistochemistry
Therapeutics
Infection

Keywords

  • Autologous transplantation
  • Duchene muscular dystrophy (DMD)
  • Dystrophin-positive fibers
  • Full-length dystrophin cDNA
  • HSV-1 amplicons

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Bujold, M., Caron, N., Camiran, G., Mukherjee, S., Allen, P. D., Tremblay, J. P., & Wang, Y. (2002). Autotransplantation in mdx mice of mdx myoblasts genetically corrected by an HSV-1 amplicon vector. Cell Transplantation, 11(8), 759-767.

Autotransplantation in mdx mice of mdx myoblasts genetically corrected by an HSV-1 amplicon vector. / Bujold, Mathieu; Caron, Nicolas; Camiran, Goeffrey; Mukherjee, Santwana; Allen, Paul D.; Tremblay, Jacques P.; Wang, Yaming.

In: Cell Transplantation, Vol. 11, No. 8, 2002, p. 759-767.

Research output: Contribution to journalArticle

Bujold, M, Caron, N, Camiran, G, Mukherjee, S, Allen, PD, Tremblay, JP & Wang, Y 2002, 'Autotransplantation in mdx mice of mdx myoblasts genetically corrected by an HSV-1 amplicon vector', Cell Transplantation, vol. 11, no. 8, pp. 759-767.
Bujold M, Caron N, Camiran G, Mukherjee S, Allen PD, Tremblay JP et al. Autotransplantation in mdx mice of mdx myoblasts genetically corrected by an HSV-1 amplicon vector. Cell Transplantation. 2002;11(8):759-767.
Bujold, Mathieu ; Caron, Nicolas ; Camiran, Goeffrey ; Mukherjee, Santwana ; Allen, Paul D. ; Tremblay, Jacques P. ; Wang, Yaming. / Autotransplantation in mdx mice of mdx myoblasts genetically corrected by an HSV-1 amplicon vector. In: Cell Transplantation. 2002 ; Vol. 11, No. 8. pp. 759-767.
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abstract = "Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder, characterized by a lack of dystrophin. To eliminate the need for immunosuppressive drugs, transplantation of genetically modified autologous myoblasts has been proposed as a possible therapy for this myopathy. An HSV-1 amplicon vector (HSVDGN), containing a 17.3-kb full-length MCK-driven mouse dystrophin cDNA, an eGFP gene, and a neomycin resistance gene driven by CMV or SV40 promoters, respectively, was constructed and used to transduce mdx primary myoblasts. The presence of the eGFP and neomycin resistance genes facilitated the evaluation of the initial transduction efficiency and the permanent transduction frequency. At low multiplicities of infection (MOI 1-5), the majority of myoblasts (60-90{\%}) expressed GFP. The GFP-positive mdx myoblasts were sorted by FACS and selected with neomycin (300 μg/ml) for 2 weeks. Up to 2{\%} of initially infected mdx myoblasts stably expressed the three transgenes without further selection at that time. These altered cells were grafted into the tibialis anterior muscles of 18 mdx mice. Some of the mice were immunosuppressed with FK506 due to the anticipation that eGFP and the product of neomycin resistance gene might be immunogenic, One month after transplantation, numerous muscle fibers expressing mouse dystrophin were detected by immunohistochemistry, in both immunosuppressed (10-50{\%}) and nonimmunosuppressed (5-25{\%}) mdx mice. Our results demonstrated the capability of permanently expressing a full-length dystrophin in dystrophic myoblasts with HSV-1 amplicon vector and raised the possibility of an eventual treatment of DMD based on the transplantation of genetically modified autologous myoblasts.",
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