Autoregulation of gene expression. Quantitative evaluation of the expression and function of the bacteriophage T4 gene 32 (single-stranded DNA binding) protein system

Peter H. von Hippel, Stephen C. Kowalczykowski, Nils Lonberg, John W. Newport, Leland S. Paul, Gary D. Stormo, Larry Gold

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The free concentration of bacteriophage T4-coded gene 32 (single-stranded DNA binding) protein in the cell is autoregulated at the translational level during T4 infection of Escherichia coli. The control of the synthesis of this protein reflects the following progression of net (co-operative) binding affinities for the various potential nucleic acid binding targets present: single-stranded DNA > gene 32 mRNA > other T4 mRNAs ≥ double-stranded DNA. In this paper we show that the free concentration of gene 32 protein is maintained at 2 to 3 μm, and use the measured binding parameters for gene 32 protein, extrapolated to intracellular conditions, to provide a quantitative molecular interpretation of this system of control of gene expression. These results are then further utilized to define the specific autoregulatory binding sequence (translational operator site) on the gene 32 mRNA as a uniquely unstructured finite binding lattice terminated by elements of secondary structure not subject to melting by gene 32 protein at the autoregulated concentration, and to predict how this site must differ from those found on other T4 messenger RNAs. It is shown that these predictions are fully consistent with available T4 DNA sequence data. The control of free protein concentration as a method of genome regulation is discussed in terms of other systems to which these approaches may apply.

Original languageEnglish (US)
Pages (from-to)795-818
Number of pages24
JournalJournal of Molecular Biology
Volume162
Issue number4
DOIs
StatePublished - Dec 25 1982
Externally publishedYes

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Bacteriophage T4
DNA-Binding Proteins
Homeostasis
Gene Expression
Messenger RNA
Genes
Proteins
Escherichia coli Infections
Single-Stranded DNA
Nucleic Acids
Freezing
Genome
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Autoregulation of gene expression. Quantitative evaluation of the expression and function of the bacteriophage T4 gene 32 (single-stranded DNA binding) protein system. / von Hippel, Peter H.; Kowalczykowski, Stephen C.; Lonberg, Nils; Newport, John W.; Paul, Leland S.; Stormo, Gary D.; Gold, Larry.

In: Journal of Molecular Biology, Vol. 162, No. 4, 25.12.1982, p. 795-818.

Research output: Contribution to journalArticle

von Hippel, Peter H. ; Kowalczykowski, Stephen C. ; Lonberg, Nils ; Newport, John W. ; Paul, Leland S. ; Stormo, Gary D. ; Gold, Larry. / Autoregulation of gene expression. Quantitative evaluation of the expression and function of the bacteriophage T4 gene 32 (single-stranded DNA binding) protein system. In: Journal of Molecular Biology. 1982 ; Vol. 162, No. 4. pp. 795-818.
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