Autoradiographic localization of osteogenin binding sites in cartilage and bone during rat embryonic development

Slobodan Vukicevic, Vishwas M. Paralkar, Noreen S. Cunningham, Jorge S. Gutkind, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Osteogenin, a novel bone differentiation factor isolated from bone, has been recently purified and the amino acid sequence determined. Osteogenin in conjunction with a collagenous bone matrix substratum induces cartilage and bone formation in vivo. In order to understand the developmental role of osteogenin during cartilage and bone morphogenesis we examined the binding and distribution of iodinated osteogenin in developing rat embryos. Whole embryo tissue sections were made from 11, 12, 13, 15, 18, and 20 day fetuses. The specific binding of osteogenin at different stages of rat embryonic development was determined by autoradiography. Maximal binding was observed in mesodermal tissues such as cartilage, bone, perichondrium, and periosteum. During Days 11-15, peak binding was localized to perichondrium during limb and vertebral morphogenesis. By Day 18 periosteum exhibited the highest concentration of autoradiographic grains. Osteogenin was also localized in developing membranous bones of the calvarium and other craniofacial bones. Considerably less binding was observed, in decreasing order, in muscle, liver, spleen, skin, brain, heart, kidney, and intestine. The observed maximal binding during skeletal morphogenesis implies a developmental role for osteogenin.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalDevelopmental Biology
Issue number1
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology


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