Autoradiographic evidence for bidirectional DNA replication in escherichia coli

R. L. Rodriguez, M. S. Dalbey, C. I. Davern

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Autoradiographic evidence is presented that demonstrates bidirectional DNA replication during a synchronous round of DNA synthesis in a culture of a reversible temperature sensitive DNA initiation mutant of Escherichia coli K12. High specific activity [3H]thymine was incorporated into the origins and termini of chromosomes which were otherwise uniformly labeled with low specific activity [3H]thymine. Autoradiographs of such differentially labeled chromosomes show two regions of high grain density symmetrically disposed on the circular chromosomes. This demonstrates that the origins and termini of replication are not contiguous; therefore replication must have proceeded in two directions.

Original languageEnglish (US)
JournalJournal of Molecular Biology
Volume74
Issue number4
DOIs
StatePublished - Mar 15 1973

ASJC Scopus subject areas

  • Virology

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