Autonomic control of bronchial blood flow and airway dimensions during strenuous exercise in sheep

A. Quail, S. McIlveen, Robert Bishop, D. McLeod, R. Gunther, J. Davis, L. Talken, D. Cottee, G. Parsons, S. White

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: During exercise and recovery the transient and steady-state changes in autonomic activity regulating lower airway blood flow and dimensions are unknown. The aim of this study was to define changes in bronchial blood flow (Qbr) and dimensions during moderate and strenuous exercise, and to analyse the role of vagal and sympathetic nerves. Methods: Nine ewes (34-44 kg) underwent left thoracotomy during general anaesthesia (thiopentone/isoflurane) and either (5 sheep=Group 1) a pulsed Doppler transducer was placed on the bronchial artery, or (4 sheep=Group 2) a pulsed Doppler transducer was placed on the bronchial artery, and transit-time and single crystal sonomicrometers were mounted on the left main bronchus. These measured continuously Qbr, bronchial circumference (Circbr) and wall thickness (Thbr). Aortic pressure (Pa) and central venous pressure catheters were placed in the superficial cervical artery and vein. Trained sheep exercised on a horizontal treadmill, i.e. Group 1, moderate exercise 2.2 mph over 1.6, 6 min recovery, for analysis of changes in Qbr before and after cholinoceptor blockade; Group 2, strenuous exercise 4.4 mph over 2, 10 min recovery for analysis of changes in Qbr and airway dimensions, before and after cholinoceptor plus α1-, α2-adrenoceptor blockade. β-adrenoceptor systems were intact. Results: In Group 1 during moderate exercise Pa and heart rate (HR) rose. Qbr and blood flow conductance (Cbr) fell immediately to 83% (P < 0.001) before returning toward resting levels, but fell when exercise ceased to 89% (P < 0.01) before recovering. Prior cholinoceptor blockade abolished the immediate fall in Qbr and Cbr, but not the recovery vasoconstriction. Later in recovery the bronchial bed dilated progressively over 6 min (P < 0.05). In Group 2 during strenuous exercise Pa and HR rose substantially. Qbr and Cbr fell to 68% and 54% (P < 0.001), respectively, and there was early vasoconstriction in recovery. Circbr fell immediately and remained at 93% (P < 0.01), and did not recover fully when exercise ceased. Thbr did not change during or after exercise. Prior cholinoceptor plus α-adrenoceptor block caused Pa and Qbr to fall slightly during exercise, but the bronchovascular constriction during and after exercise was abolished, as was circumferential shortening in the airway. Conclusions: At exercise onset and steady-state, resetting the arterial baroreflex upward in sheep increases parasympathetic cholinergic vasoconstrictor activity and causes bronchial wall and bronchovascular smooth muscle contraction in concert with sympathetic adrenergic constriction of systemic vascular beds. Whether the known sigmoid baroreflex control of tracheal smooth muscle tension at rest is extended to tracheobronchial smooth muscle and its circulation during exercise is yet to be determined.

Original languageEnglish (US)
Pages (from-to)190-199
Number of pages10
JournalPulmonary Pharmacology and Therapeutics
Volume20
Issue number2
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

Fingerprint

Cholinergic Receptors
Sheep
Blood
Adrenergic Receptors
Bronchial Arteries
Smooth Muscle
Recovery
Baroreflex
Vasoconstriction
Transducers
Constriction
Muscle
Heart Rate
Muscle Tonus
Central Venous Pressure
Thiopental
Central Venous Catheters
Isoflurane
Vasoconstrictor Agents
Sigmoid Colon

Keywords

  • Airway circumference
  • Airway wall thickness
  • Alpha-adrenoceptors
  • Autonomic control
  • Awake sheep
  • Baroreflex resetting
  • Bronchial blood flow
  • Cholinoceptors
  • Exercise
  • Sonomicrometry
  • Sympathetic nerves
  • Vagus nerve

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Autonomic control of bronchial blood flow and airway dimensions during strenuous exercise in sheep. / Quail, A.; McIlveen, S.; Bishop, Robert; McLeod, D.; Gunther, R.; Davis, J.; Talken, L.; Cottee, D.; Parsons, G.; White, S.

In: Pulmonary Pharmacology and Therapeutics, Vol. 20, No. 2, 01.04.2007, p. 190-199.

Research output: Contribution to journalArticle

Quail, A, McIlveen, S, Bishop, R, McLeod, D, Gunther, R, Davis, J, Talken, L, Cottee, D, Parsons, G & White, S 2007, 'Autonomic control of bronchial blood flow and airway dimensions during strenuous exercise in sheep', Pulmonary Pharmacology and Therapeutics, vol. 20, no. 2, pp. 190-199. https://doi.org/10.1016/j.pupt.2006.03.010
Quail, A. ; McIlveen, S. ; Bishop, Robert ; McLeod, D. ; Gunther, R. ; Davis, J. ; Talken, L. ; Cottee, D. ; Parsons, G. ; White, S. / Autonomic control of bronchial blood flow and airway dimensions during strenuous exercise in sheep. In: Pulmonary Pharmacology and Therapeutics. 2007 ; Vol. 20, No. 2. pp. 190-199.
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TY - JOUR

T1 - Autonomic control of bronchial blood flow and airway dimensions during strenuous exercise in sheep

AU - Quail, A.

AU - McIlveen, S.

AU - Bishop, Robert

AU - McLeod, D.

AU - Gunther, R.

AU - Davis, J.

AU - Talken, L.

AU - Cottee, D.

AU - Parsons, G.

AU - White, S.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Background: During exercise and recovery the transient and steady-state changes in autonomic activity regulating lower airway blood flow and dimensions are unknown. The aim of this study was to define changes in bronchial blood flow (Qbr) and dimensions during moderate and strenuous exercise, and to analyse the role of vagal and sympathetic nerves. Methods: Nine ewes (34-44 kg) underwent left thoracotomy during general anaesthesia (thiopentone/isoflurane) and either (5 sheep=Group 1) a pulsed Doppler transducer was placed on the bronchial artery, or (4 sheep=Group 2) a pulsed Doppler transducer was placed on the bronchial artery, and transit-time and single crystal sonomicrometers were mounted on the left main bronchus. These measured continuously Qbr, bronchial circumference (Circbr) and wall thickness (Thbr). Aortic pressure (Pa) and central venous pressure catheters were placed in the superficial cervical artery and vein. Trained sheep exercised on a horizontal treadmill, i.e. Group 1, moderate exercise 2.2 mph over 1.6, 6 min recovery, for analysis of changes in Qbr before and after cholinoceptor blockade; Group 2, strenuous exercise 4.4 mph over 2, 10 min recovery for analysis of changes in Qbr and airway dimensions, before and after cholinoceptor plus α1-, α2-adrenoceptor blockade. β-adrenoceptor systems were intact. Results: In Group 1 during moderate exercise Pa and heart rate (HR) rose. Qbr and blood flow conductance (Cbr) fell immediately to 83% (P < 0.001) before returning toward resting levels, but fell when exercise ceased to 89% (P < 0.01) before recovering. Prior cholinoceptor blockade abolished the immediate fall in Qbr and Cbr, but not the recovery vasoconstriction. Later in recovery the bronchial bed dilated progressively over 6 min (P < 0.05). In Group 2 during strenuous exercise Pa and HR rose substantially. Qbr and Cbr fell to 68% and 54% (P < 0.001), respectively, and there was early vasoconstriction in recovery. Circbr fell immediately and remained at 93% (P < 0.01), and did not recover fully when exercise ceased. Thbr did not change during or after exercise. Prior cholinoceptor plus α-adrenoceptor block caused Pa and Qbr to fall slightly during exercise, but the bronchovascular constriction during and after exercise was abolished, as was circumferential shortening in the airway. Conclusions: At exercise onset and steady-state, resetting the arterial baroreflex upward in sheep increases parasympathetic cholinergic vasoconstrictor activity and causes bronchial wall and bronchovascular smooth muscle contraction in concert with sympathetic adrenergic constriction of systemic vascular beds. Whether the known sigmoid baroreflex control of tracheal smooth muscle tension at rest is extended to tracheobronchial smooth muscle and its circulation during exercise is yet to be determined.

AB - Background: During exercise and recovery the transient and steady-state changes in autonomic activity regulating lower airway blood flow and dimensions are unknown. The aim of this study was to define changes in bronchial blood flow (Qbr) and dimensions during moderate and strenuous exercise, and to analyse the role of vagal and sympathetic nerves. Methods: Nine ewes (34-44 kg) underwent left thoracotomy during general anaesthesia (thiopentone/isoflurane) and either (5 sheep=Group 1) a pulsed Doppler transducer was placed on the bronchial artery, or (4 sheep=Group 2) a pulsed Doppler transducer was placed on the bronchial artery, and transit-time and single crystal sonomicrometers were mounted on the left main bronchus. These measured continuously Qbr, bronchial circumference (Circbr) and wall thickness (Thbr). Aortic pressure (Pa) and central venous pressure catheters were placed in the superficial cervical artery and vein. Trained sheep exercised on a horizontal treadmill, i.e. Group 1, moderate exercise 2.2 mph over 1.6, 6 min recovery, for analysis of changes in Qbr before and after cholinoceptor blockade; Group 2, strenuous exercise 4.4 mph over 2, 10 min recovery for analysis of changes in Qbr and airway dimensions, before and after cholinoceptor plus α1-, α2-adrenoceptor blockade. β-adrenoceptor systems were intact. Results: In Group 1 during moderate exercise Pa and heart rate (HR) rose. Qbr and blood flow conductance (Cbr) fell immediately to 83% (P < 0.001) before returning toward resting levels, but fell when exercise ceased to 89% (P < 0.01) before recovering. Prior cholinoceptor blockade abolished the immediate fall in Qbr and Cbr, but not the recovery vasoconstriction. Later in recovery the bronchial bed dilated progressively over 6 min (P < 0.05). In Group 2 during strenuous exercise Pa and HR rose substantially. Qbr and Cbr fell to 68% and 54% (P < 0.001), respectively, and there was early vasoconstriction in recovery. Circbr fell immediately and remained at 93% (P < 0.01), and did not recover fully when exercise ceased. Thbr did not change during or after exercise. Prior cholinoceptor plus α-adrenoceptor block caused Pa and Qbr to fall slightly during exercise, but the bronchovascular constriction during and after exercise was abolished, as was circumferential shortening in the airway. Conclusions: At exercise onset and steady-state, resetting the arterial baroreflex upward in sheep increases parasympathetic cholinergic vasoconstrictor activity and causes bronchial wall and bronchovascular smooth muscle contraction in concert with sympathetic adrenergic constriction of systemic vascular beds. Whether the known sigmoid baroreflex control of tracheal smooth muscle tension at rest is extended to tracheobronchial smooth muscle and its circulation during exercise is yet to be determined.

KW - Airway circumference

KW - Airway wall thickness

KW - Alpha-adrenoceptors

KW - Autonomic control

KW - Awake sheep

KW - Baroreflex resetting

KW - Bronchial blood flow

KW - Cholinoceptors

KW - Exercise

KW - Sonomicrometry

KW - Sympathetic nerves

KW - Vagus nerve

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