Automated quantification of choriocapillaris anatomical features in ultrahigh-speed optical coherence tomography angiograms

Brennan Marsh-Armstrong, Justin Migacz, Ravi Jonnal, John S. Werner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


In vivo visualization and quantification of choriocapillaris vascular anatomy is a fundamental step in understanding the relation between choriocapillaris degradation and atrophic retinopathies, including geographic atrophy. We describe a process utilizing ultrahigh-speed swept-source optical coherence tomography and a custom-designed “local min-max normalized masking” algorithm to extract in vivo anatomical metrics of the choriocapillaris. We used a swept-source optical coherence tomography system with a 1.6 MHz A-scan rate to image healthy retinas. With the postprocessing algorithm, we reduced noise, optimized visibility of vasculature, and skeletonized the vasculature within the images. These skeletonizations were in 89% agreement with those made by skilled technicians and were, on average, completed in 18.6 s as compared to the 5.6 h technicians required. Anatomy within the processed images and skeletonizations was analyzed to identify average values (mean±SD) of flow void radius (9.8 ± 0.7 µm), flow void area (749 ± 110 µm2), vessel radius (5.0 ± 0.3 µm), branch-point to branch-point vessel length (26.8 ± 1.1 µm), and branches per branch-point (3.1 ± 0.1). To exemplify the uses of this tool a retina with geographic atrophy was imaged and processed to reveal statistically significant (p<0.05) increases in flow void radii and decreases in vessel radii under atrophic lesions as compared to atrophy-free regions on the same retina. Our results demonstrate a new avenue for quantifying choriocapillaris anatomy and studying vasculature changes in atrophic retinopathies.

Original languageEnglish (US)
Pages (from-to)5337-5350
Number of pages14
JournalBiomedical Optics Express
Issue number10
StatePublished - Oct 1 2019

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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