Automated computation of functional vascular density using laser speckle imaging in a rodent window chamber model

Sean M. White, Steven George, Bernard Choi

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

We report a methodology for computing functional vascular density within a rodent dorsal window chamber model based on long-exposure laser speckle imaging (LSI). This technique relies on the presence of flow to create detailed vasculature maps. Employing this contrast mechanism is not possible using conventional imaging methods. Additionally, a freeware algorithm for computing functional vascular density (FVD) from images acquired using long-exposure LSI is also described to facilitate ease in adopting this method. We demonstrate that together these tools can be used to compute FVD nearly twelve times faster than manual computation, yet with comparable accuracy.

Original languageEnglish (US)
Pages (from-to)92-95
Number of pages4
JournalMicrovascular Research
Volume82
Issue number1
DOIs
StatePublished - Jul 1 2011

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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