Autologous myoblasts attenuate atrophy and improve tongue force in a denervated tongue model

A pilot study

Emily K. Plowman, Khadijeh Bijangi-Vishehsaraei, Stacey Halum, Daniel Cates, Helmut Hanenberg, Amanda S. Domer, Jan Nolta, Peter C Belafsky

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives/Hypothesis Autologous muscle-derived stem cell (MdSC) therapy is a promising treatment to restore function. No group has evaluated MdSC therapy in a denervated tongue model. The purpose of this pilot investigation was to determine the extent of autologous MdSC survival, effects on tongue muscle atrophy, maximal contractile force, and lingual pressure in a denervated ovine tongue model. Study Design Pilot animal experiment. Methods Bilateral implantable cuff electrodes were placed around the hypoglossal nerves in two Dorper cross ewes. Tensometer and high-resolution manometry (HRM) testing were performed during supermaximum hypoglossal nerve stimulation to assess baseline tongue strength. Sternocleidomastoid muscle biopsies were acquired to create autologous MdSC cultures. At 1 month, 5 × 108 green fluorescent protein (GFP)-labeled autologous MdSCs were injected into the partially denervated tongue. Two-months postinjection, lingual tensometer testing, HRM, and postmortem histological assessment were performed. Results GFP+ myofibers were identified in denervated tongue specimens indicating MdSC survival. Muscle fiber diameter was larger in GFP+ fibers for both tongue specimens, suggesting attenuation of muscle atrophy. Myofiber diameter was larger in GFP+ myofibers than preinjury diameters, providing evidence of new muscle formation. These myogenic changes led to a 27% increase in maximal tongue contractile force and a 54% increase in maximum base of tongue pressure in one animal. Conclusions Autologous MdSC therapy may be a viable treatment for the partially denervated tongue, with current findings demonstrating that injected MdSCs survived and fused with tongue myofibers, with a resultant increase in myofiber diameter and an increase in tongue strength. Level of Evidence N/A.

Original languageEnglish (US)
JournalLaryngoscope
Volume124
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Myoblasts
Tongue
Atrophy
Muscles
Stem Cells
Green Fluorescent Proteins
Cell- and Tissue-Based Therapy
Hypoglossal Nerve
Muscular Atrophy
Manometry
Cell Survival
Pressure
Implanted Electrodes
Sheep

Keywords

  • autologous
  • denervated tongue
  • dysphagia
  • muscle-derived stem cells
  • myoblast
  • myofibers
  • oropharyngeal dysphagia
  • satellite cells
  • Stem cells
  • tongue
  • tongue paralysis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Autologous myoblasts attenuate atrophy and improve tongue force in a denervated tongue model : A pilot study. / Plowman, Emily K.; Bijangi-Vishehsaraei, Khadijeh; Halum, Stacey; Cates, Daniel; Hanenberg, Helmut; Domer, Amanda S.; Nolta, Jan; Belafsky, Peter C.

In: Laryngoscope, Vol. 124, No. 2, 02.2014.

Research output: Contribution to journalArticle

Plowman, Emily K. ; Bijangi-Vishehsaraei, Khadijeh ; Halum, Stacey ; Cates, Daniel ; Hanenberg, Helmut ; Domer, Amanda S. ; Nolta, Jan ; Belafsky, Peter C. / Autologous myoblasts attenuate atrophy and improve tongue force in a denervated tongue model : A pilot study. In: Laryngoscope. 2014 ; Vol. 124, No. 2.
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abstract = "Objectives/Hypothesis Autologous muscle-derived stem cell (MdSC) therapy is a promising treatment to restore function. No group has evaluated MdSC therapy in a denervated tongue model. The purpose of this pilot investigation was to determine the extent of autologous MdSC survival, effects on tongue muscle atrophy, maximal contractile force, and lingual pressure in a denervated ovine tongue model. Study Design Pilot animal experiment. Methods Bilateral implantable cuff electrodes were placed around the hypoglossal nerves in two Dorper cross ewes. Tensometer and high-resolution manometry (HRM) testing were performed during supermaximum hypoglossal nerve stimulation to assess baseline tongue strength. Sternocleidomastoid muscle biopsies were acquired to create autologous MdSC cultures. At 1 month, 5 × 108 green fluorescent protein (GFP)-labeled autologous MdSCs were injected into the partially denervated tongue. Two-months postinjection, lingual tensometer testing, HRM, and postmortem histological assessment were performed. Results GFP+ myofibers were identified in denervated tongue specimens indicating MdSC survival. Muscle fiber diameter was larger in GFP+ fibers for both tongue specimens, suggesting attenuation of muscle atrophy. Myofiber diameter was larger in GFP+ myofibers than preinjury diameters, providing evidence of new muscle formation. These myogenic changes led to a 27{\%} increase in maximal tongue contractile force and a 54{\%} increase in maximum base of tongue pressure in one animal. Conclusions Autologous MdSC therapy may be a viable treatment for the partially denervated tongue, with current findings demonstrating that injected MdSCs survived and fused with tongue myofibers, with a resultant increase in myofiber diameter and an increase in tongue strength. Level of Evidence N/A.",
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AU - Plowman, Emily K.

AU - Bijangi-Vishehsaraei, Khadijeh

AU - Halum, Stacey

AU - Cates, Daniel

AU - Hanenberg, Helmut

AU - Domer, Amanda S.

AU - Nolta, Jan

AU - Belafsky, Peter C

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AB - Objectives/Hypothesis Autologous muscle-derived stem cell (MdSC) therapy is a promising treatment to restore function. No group has evaluated MdSC therapy in a denervated tongue model. The purpose of this pilot investigation was to determine the extent of autologous MdSC survival, effects on tongue muscle atrophy, maximal contractile force, and lingual pressure in a denervated ovine tongue model. Study Design Pilot animal experiment. Methods Bilateral implantable cuff electrodes were placed around the hypoglossal nerves in two Dorper cross ewes. Tensometer and high-resolution manometry (HRM) testing were performed during supermaximum hypoglossal nerve stimulation to assess baseline tongue strength. Sternocleidomastoid muscle biopsies were acquired to create autologous MdSC cultures. At 1 month, 5 × 108 green fluorescent protein (GFP)-labeled autologous MdSCs were injected into the partially denervated tongue. Two-months postinjection, lingual tensometer testing, HRM, and postmortem histological assessment were performed. Results GFP+ myofibers were identified in denervated tongue specimens indicating MdSC survival. Muscle fiber diameter was larger in GFP+ fibers for both tongue specimens, suggesting attenuation of muscle atrophy. Myofiber diameter was larger in GFP+ myofibers than preinjury diameters, providing evidence of new muscle formation. These myogenic changes led to a 27% increase in maximal tongue contractile force and a 54% increase in maximum base of tongue pressure in one animal. Conclusions Autologous MdSC therapy may be a viable treatment for the partially denervated tongue, with current findings demonstrating that injected MdSCs survived and fused with tongue myofibers, with a resultant increase in myofiber diameter and an increase in tongue strength. Level of Evidence N/A.

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KW - tongue paralysis

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