Autofluorescence dynamics during reperfusion following long-term renal ischemia in a rat model

Rajesh N. Raman, Christopher D. Pivetti, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical properties of near-surface kidney tissue were monitored in order to assess response during reperfusion to long (20 minutes) versus prolonged (150 minutes) ischemia in an in vivo rat model. Specifically, autofluorescence images of the exposed surfaces of both the normal and the ischemic kidneys were acquired during both injury and reperfusion alternately under 355 nm and 266 nm excitations. The temporal profile of the emission of the injured kidney during the reperfusion phase under 355 nm excitation was normalized to that under 266 nm as a means to account for changes in tissue optical properties independent of ischemia as well as changes in the illumination/collection geometrical parameters in future clinical implementation of this technique using a hand-held probe. The scattered excitation light signal was also evaluated as a reference signal and found to be inadequate. Characteristic time constants were extracted using a fit to a relaxation model and found to have larger mean values following 150 minutes of injury. The mean values were then compared with the outcome of a chronic survival study where the control kidney had been removed. Rat kidneys exhibiting longer time constants were much more likely to fail. This may lead to a method to assess kidney viability and predict its ability to recover in the initial period following transplantation or resuscitation.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6853
DOIs
StatePublished - 2008
EventBiomedical Optical Spectroscopy - San Jose, CA, United States
Duration: Jan 19 2008Jan 23 2008

Other

OtherBiomedical Optical Spectroscopy
CountryUnited States
CitySan Jose, CA
Period1/19/081/23/08

Keywords

  • Autofluorescence
  • In vivo
  • Ischemia
  • Kidney
  • Organ transplantation
  • Rat
  • Reperfusion
  • Tissue viability

ASJC Scopus subject areas

  • Engineering(all)

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