Autofluorescence-based analyses of intranuclear inclusions of Fragile X-associated tremor/ataxia syndrome

Research output: Contribution to journalArticle

Abstract

Intranuclear inclusions present in the brains of patients with Fragile X-associated tremor/ataxia syndrome (FXTAS) have historically been difficult to study due to their location and scarcity. The recent finding that these particles autofluoresce has complicated the use of immunofluorescence techniques, but also offers new opportunities for purification. We have ascertained the features of the autofluorescence, including its excitation/emission spectrum, similarities and differences compared with lipofuscin autofluorescence, and its presence/absence under various fixation, mounting and UV light exposure conditions. Immunofluorescence at various wavelengths was conducted to determine which conditions are ideal forminimizing autofluorescence confounds. We also present a technique for autofluorescence-based sorting of FXTAS inclusions using flow cytometry, which will allow researchers in the field to purify inclusions more successfully for unbiased analyses.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalBioTechniques
Volume69
Issue number1
DOIs
StatePublished - Apr 2020

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Autofluorescence-based analyses of intranuclear inclusions of Fragile X-associated tremor/ataxia syndrome'. Together they form a unique fingerprint.

  • Cite this