Abstract
Background: Oxidation of low-density lipoprotein (LDL) is a crucial step in atherogenesis. There is an urgent need for direct measures of in vivo oxidative stress. Autoantibodies against oxidized low-density lipoprotein (Ab against Ox-LDL) are a direct measure of oxidative stress and predict cardiovascular disease. Our aim was to evaluate an ELISA for Ab against Ox-LDL in Type 2 diabetes, a condition with increased oxidative stress. Methods: Ab against Ox-LDL were measured by ELISA and expressed as a ratio of Ox-LDL to native LDL (N-LDL). Samples were obtained from 45 Type 2 diabetic patients and 25 matched controls before and after supplementation with alpha tocopherol (AT, 1200 IU/day). Results: The assay had good precision. While there was no interference with bilirubin and hemolysis, triglycerides 500 mg/dl increased antibody titer, which was abrogated by airfuging. Compared to controls, significantly increased titers of Ab against Ox-LDL were found in diabetics (diabetes mellitus Type 2) with macrovascular disease (DM2-MV), but not without macrovascular disease (DM2) (DM2: 1.32 ± 0.33; DM2-MV: 1.48 ± 0.44 vs. controls, 1.21 ± 0.28; p < 0.05). AT supplementation significantly decreased titers of Ab against Ox-LDL in both diabetic groups (p < 0.01). Conclusion: This assay may serve as a future test for the assessment of cardiovascular risk especially in patients with increased oxidative stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-150 |
| Number of pages | 6 |
| Journal | Clinica Chimica Acta |
| Volume | 317 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Autoantibodies against oxidized low-density lipoprotein
- Immunoassay
- Type 2 diabetes mellitus
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
Cite this
Autoantibodies to oxidized low-density lipoprotein in patients with Type 2 diabetes mellitus. / Hsu, Rebecca M.; Devaraj, Sridevi; Jialal, Ishwarlal.
In: Clinica Chimica Acta, Vol. 317, No. 1-2, 2002, p. 145-150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Autoantibodies to oxidized low-density lipoprotein in patients with Type 2 diabetes mellitus
AU - Hsu, Rebecca M.
AU - Devaraj, Sridevi
AU - Jialal, Ishwarlal
PY - 2002
Y1 - 2002
N2 - Background: Oxidation of low-density lipoprotein (LDL) is a crucial step in atherogenesis. There is an urgent need for direct measures of in vivo oxidative stress. Autoantibodies against oxidized low-density lipoprotein (Ab against Ox-LDL) are a direct measure of oxidative stress and predict cardiovascular disease. Our aim was to evaluate an ELISA for Ab against Ox-LDL in Type 2 diabetes, a condition with increased oxidative stress. Methods: Ab against Ox-LDL were measured by ELISA and expressed as a ratio of Ox-LDL to native LDL (N-LDL). Samples were obtained from 45 Type 2 diabetic patients and 25 matched controls before and after supplementation with alpha tocopherol (AT, 1200 IU/day). Results: The assay had good precision. While there was no interference with bilirubin and hemolysis, triglycerides 500 mg/dl increased antibody titer, which was abrogated by airfuging. Compared to controls, significantly increased titers of Ab against Ox-LDL were found in diabetics (diabetes mellitus Type 2) with macrovascular disease (DM2-MV), but not without macrovascular disease (DM2) (DM2: 1.32 ± 0.33; DM2-MV: 1.48 ± 0.44 vs. controls, 1.21 ± 0.28; p < 0.05). AT supplementation significantly decreased titers of Ab against Ox-LDL in both diabetic groups (p < 0.01). Conclusion: This assay may serve as a future test for the assessment of cardiovascular risk especially in patients with increased oxidative stress.
AB - Background: Oxidation of low-density lipoprotein (LDL) is a crucial step in atherogenesis. There is an urgent need for direct measures of in vivo oxidative stress. Autoantibodies against oxidized low-density lipoprotein (Ab against Ox-LDL) are a direct measure of oxidative stress and predict cardiovascular disease. Our aim was to evaluate an ELISA for Ab against Ox-LDL in Type 2 diabetes, a condition with increased oxidative stress. Methods: Ab against Ox-LDL were measured by ELISA and expressed as a ratio of Ox-LDL to native LDL (N-LDL). Samples were obtained from 45 Type 2 diabetic patients and 25 matched controls before and after supplementation with alpha tocopherol (AT, 1200 IU/day). Results: The assay had good precision. While there was no interference with bilirubin and hemolysis, triglycerides 500 mg/dl increased antibody titer, which was abrogated by airfuging. Compared to controls, significantly increased titers of Ab against Ox-LDL were found in diabetics (diabetes mellitus Type 2) with macrovascular disease (DM2-MV), but not without macrovascular disease (DM2) (DM2: 1.32 ± 0.33; DM2-MV: 1.48 ± 0.44 vs. controls, 1.21 ± 0.28; p < 0.05). AT supplementation significantly decreased titers of Ab against Ox-LDL in both diabetic groups (p < 0.01). Conclusion: This assay may serve as a future test for the assessment of cardiovascular risk especially in patients with increased oxidative stress.
KW - Autoantibodies against oxidized low-density lipoprotein
KW - Immunoassay
KW - Type 2 diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=0036157629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036157629&partnerID=8YFLogxK
U2 - 10.1016/S0009-8981(01)00767-7
DO - 10.1016/S0009-8981(01)00767-7
M3 - Article
C2 - 11814469
AN - SCOPUS:0036157629
VL - 317
SP - 145
EP - 150
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 1-2
ER -