Abstract
Autism is a complex neurodevelopmental disorder with unknown etiology. One hypothesis regarding etiology in autism is the "opioid peptide excess" theory that postulates that excessive amounts of exogenous opioid-like peptides derived from dietary proteins are detectable in urine and that these compounds may be pathophysiologically important in autism. A selective LC-MS/MS method was developed to analyze gliadinomorphin, β-casomorphin, deltorphin 1, and deltorphin 2 in urine. The method is based on on-line SPE extraction of the neuropeptides from urine, column switching, and subsequent HPLC analysis. A limit of detection of 0.25 ng/mL was achieved for all analytes. Analyte recovery rates from urine ranged between 78% and 94%, with relative standard deviations of 0.2-6.8%. The method was used to screen 69 urine samples from children with and without autism spectrum disorders for the occurrence of neuropeptides. The target neuropeptides were not detected above the detection limit in either sample set.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1643-1651 |
| Number of pages | 9 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 388 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2007 |
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Keywords
- β-Casomorphin
- Autism
- Gliadinomorphin
- Neuropeptides
- On-line SPE-HPLC-MS/MS
- Opioid peptide excess theory
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
Cite this
Autism and urinary exogenous neuropeptides : Development of an on-line SPE-HPLC-tandem mass spectrometry method to test the opioid excess theory. / Dettmer, K.; Hanna, D.; Whetstone, P.; Hansen, Robin L; Hammock, B. D.
In: Analytical and Bioanalytical Chemistry, Vol. 388, No. 8, 08.2007, p. 1643-1651.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Autism and urinary exogenous neuropeptides
T2 - Development of an on-line SPE-HPLC-tandem mass spectrometry method to test the opioid excess theory
AU - Dettmer, K.
AU - Hanna, D.
AU - Whetstone, P.
AU - Hansen, Robin L
AU - Hammock, B. D.
PY - 2007/8
Y1 - 2007/8
N2 - Autism is a complex neurodevelopmental disorder with unknown etiology. One hypothesis regarding etiology in autism is the "opioid peptide excess" theory that postulates that excessive amounts of exogenous opioid-like peptides derived from dietary proteins are detectable in urine and that these compounds may be pathophysiologically important in autism. A selective LC-MS/MS method was developed to analyze gliadinomorphin, β-casomorphin, deltorphin 1, and deltorphin 2 in urine. The method is based on on-line SPE extraction of the neuropeptides from urine, column switching, and subsequent HPLC analysis. A limit of detection of 0.25 ng/mL was achieved for all analytes. Analyte recovery rates from urine ranged between 78% and 94%, with relative standard deviations of 0.2-6.8%. The method was used to screen 69 urine samples from children with and without autism spectrum disorders for the occurrence of neuropeptides. The target neuropeptides were not detected above the detection limit in either sample set.
AB - Autism is a complex neurodevelopmental disorder with unknown etiology. One hypothesis regarding etiology in autism is the "opioid peptide excess" theory that postulates that excessive amounts of exogenous opioid-like peptides derived from dietary proteins are detectable in urine and that these compounds may be pathophysiologically important in autism. A selective LC-MS/MS method was developed to analyze gliadinomorphin, β-casomorphin, deltorphin 1, and deltorphin 2 in urine. The method is based on on-line SPE extraction of the neuropeptides from urine, column switching, and subsequent HPLC analysis. A limit of detection of 0.25 ng/mL was achieved for all analytes. Analyte recovery rates from urine ranged between 78% and 94%, with relative standard deviations of 0.2-6.8%. The method was used to screen 69 urine samples from children with and without autism spectrum disorders for the occurrence of neuropeptides. The target neuropeptides were not detected above the detection limit in either sample set.
KW - β-Casomorphin
KW - Autism
KW - Gliadinomorphin
KW - Neuropeptides
KW - On-line SPE-HPLC-MS/MS
KW - Opioid peptide excess theory
UR - http://www.scopus.com/inward/record.url?scp=34547463516&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547463516&partnerID=8YFLogxK
U2 - 10.1007/s00216-007-1301-4
DO - 10.1007/s00216-007-1301-4
M3 - Article
C2 - 17520243
AN - SCOPUS:34547463516
VL - 388
SP - 1643
EP - 1651
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 8
ER -