Abstract
Objective: To reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP). Methods: Materialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness. Results: The 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans. Conclusion: Auricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties. Levels of Evidence: NA. Laryngoscope, 2019.
| Original language | English (US) |
|---|---|
| Journal | Laryngoscope |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- 3D printing
- auricle. reconstruction
- Diced cartilage
- platelet-rich plasma
- porous hollow mold
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Auricle shaping using 3D printing and autologous diced cartilage. / Liao, Junlin; Chen, Yong; Chen, Jia; He, Bin; Qian, Li; Xu, Jiaqin; Wang, Aijun; Li, Qingfeng; Xie, Hongju; Zhou, Jianda.
In: Laryngoscope, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Auricle shaping using 3D printing and autologous diced cartilage
AU - Liao, Junlin
AU - Chen, Yong
AU - Chen, Jia
AU - He, Bin
AU - Qian, Li
AU - Xu, Jiaqin
AU - Wang, Aijun
AU - Li, Qingfeng
AU - Xie, Hongju
AU - Zhou, Jianda
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Objective: To reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP). Methods: Materialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness. Results: The 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans. Conclusion: Auricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties. Levels of Evidence: NA. Laryngoscope, 2019.
AB - Objective: To reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP). Methods: Materialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness. Results: The 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans. Conclusion: Auricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties. Levels of Evidence: NA. Laryngoscope, 2019.
KW - 3D printing
KW - auricle. reconstruction
KW - Diced cartilage
KW - platelet-rich plasma
KW - porous hollow mold
UR - http://www.scopus.com/inward/record.url?scp=85062787667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062787667&partnerID=8YFLogxK
U2 - 10.1002/lary.27752
DO - 10.1002/lary.27752
M3 - Article
JO - Laryngoscope
JF - Laryngoscope
SN - 0023-852X
ER -