Atypical chlamydiaceae in wild populations of hawks (Buteo spp.) in California

Charlene Luján-Vega, Michelle Hawkins, Christine K Johnson, Christopher Briggs, Chris Vennum, Peter H. Bloom, Joshua M. Hull, Carolyn Cray, Denise Pesti, Lisa Johnson, Paula Ciembor, Branson R. Ritchie

Research output: Contribution to journalArticle

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Abstract

Chlamydiaceae bacteria infect many vertebrate hosts, and previous reports based on polymerase chain reaction (PCR) assays and serologic assays that are prone to cross-reaction among chlamydial organisms have been used to describe the prevalence of either DNA fragments or antibodies to Chlamydia spp. in wild raptorial populations. This study reports the PCR-based prevalence of Chlamydiaceae DNA that does not 100% match any avian or mammalian Chlamydiaceae in wild populations of hawks in California Buteo species. In this study, multimucosal swab samples (n = 291) for quantitative PCR (qPCR) and plasma (n = 78) for serology were collected from wild hawks. All available plasma samples were negative for antibodies using a C. psittaci-specific elementary body agglutination test (EBA; n = 78). For IgY antibodies all 51 available samples were negative using the indirect immunofluorescent assay. The overall prevalence of Chlamydiaceae DNA detection in wild Buteo species sampled was 1.37% (4/291) via qPCR-based analysis. Two fledgling Swainson's hawks (Buteo swainsoni) and two juvenile red-tailed hawks (Buteo jamaicensis) were positive by qPCR-based assay for an atypical chlamydial sequence that did not 100% match any known C. psittaci genotype. Positive swab samples from these four birds were sequenced based on the ompA gene and compared by high-resolution melt analysis with all known avian and mammalian Chlamydiaceae. The amplicon sequence did not 100% match any known avian chlamydial sequence; however, it was most similar (98.6%) to C. psittaci M56, a genotype that is typically found in muskrats and hares. Culture and full genome sequence analysis of Chlamydia spp. isolated from diseased hawks will be necessary to classify this organism and to better understand its epizootiology and potential health impact on wild Buteo populations in California.

Original languageEnglish (US)
Pages (from-to)108-115
Number of pages8
JournalJournal of Zoo and Wildlife Medicine
Volume49
Issue number1
DOIs
StatePublished - Mar 1 2018

Fingerprint

Chlamydiaceae
Hawks
Buteo
hawks
Polymerase Chain Reaction
Buteo jamaicensis
quantitative polymerase chain reaction
Chlamydia
Population
antibodies
DNA
assays
polymerase chain reaction
Genotype
Ondatra zibethicus
Hares
sampling
Immunoglobulin Fragments
Agglutination Tests
Arvicolinae

Keywords

  • Buteo spp.
  • Chlamydiaceae
  • EBA
  • Genotype
  • IFA
  • QPCR

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Atypical chlamydiaceae in wild populations of hawks (Buteo spp.) in California. / Luján-Vega, Charlene; Hawkins, Michelle; Johnson, Christine K; Briggs, Christopher; Vennum, Chris; Bloom, Peter H.; Hull, Joshua M.; Cray, Carolyn; Pesti, Denise; Johnson, Lisa; Ciembor, Paula; Ritchie, Branson R.

In: Journal of Zoo and Wildlife Medicine, Vol. 49, No. 1, 01.03.2018, p. 108-115.

Research output: Contribution to journalArticle

Luján-Vega, C, Hawkins, M, Johnson, CK, Briggs, C, Vennum, C, Bloom, PH, Hull, JM, Cray, C, Pesti, D, Johnson, L, Ciembor, P & Ritchie, BR 2018, 'Atypical chlamydiaceae in wild populations of hawks (Buteo spp.) in California', Journal of Zoo and Wildlife Medicine, vol. 49, no. 1, pp. 108-115. https://doi.org/10.1638/2017-0053R.1
Luján-Vega, Charlene ; Hawkins, Michelle ; Johnson, Christine K ; Briggs, Christopher ; Vennum, Chris ; Bloom, Peter H. ; Hull, Joshua M. ; Cray, Carolyn ; Pesti, Denise ; Johnson, Lisa ; Ciembor, Paula ; Ritchie, Branson R. / Atypical chlamydiaceae in wild populations of hawks (Buteo spp.) in California. In: Journal of Zoo and Wildlife Medicine. 2018 ; Vol. 49, No. 1. pp. 108-115.
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