Abstract
Quantification of specific proteins depends on separation by chromatography or electrophoresis followed by chemical detection schemes such as staining and fluorophore adhesion. Chemical exchange of short-lived isotopes, particularly sulfur, is also prevalent despite the inconveniences of counting radioactivity. Physical methods based on isotopic and elemental analyses offer highly sensitive protein quantitation that has linear response over wide dynamic ranges and is independent of protein conformation. Accelerator mass spectrometry quantifies long-lived isotopes such as 14C to sub-attomole sensitivity. We quantified protein interactions with small molecules such as toxins, vitamins, and natural biochemicals at precisions of 1-5%. Micro-proton-induced X-ray emission quantifies elemental abundances in separated metalloprotein samples to nanogram amounts and is capable of quantifying phosphorylated loci in gels. Accelerator-based quantitation is a possible tool for quantifying the genome translation into proteome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2037-2045 |
| Number of pages | 9 |
| Journal | Electrophoresis |
| Volume | 22 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Accelerator mass spectrometry
- Protein
- Proteome
- Quantitation
- Radioisotope
- Review
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Attomole quantitation of protein separations with accelerator mass spectrometry. / Vogel, John S.; Grant, Patrick G.; Buchholz, Bruce A.; Dingley, Karen; Turteltaub, Ken W.
In: Electrophoresis, Vol. 22, No. 10, 2001, p. 2037-2045.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Attomole quantitation of protein separations with accelerator mass spectrometry
AU - Vogel, John S.
AU - Grant, Patrick G.
AU - Buchholz, Bruce A.
AU - Dingley, Karen
AU - Turteltaub, Ken W
PY - 2001
Y1 - 2001
N2 - Quantification of specific proteins depends on separation by chromatography or electrophoresis followed by chemical detection schemes such as staining and fluorophore adhesion. Chemical exchange of short-lived isotopes, particularly sulfur, is also prevalent despite the inconveniences of counting radioactivity. Physical methods based on isotopic and elemental analyses offer highly sensitive protein quantitation that has linear response over wide dynamic ranges and is independent of protein conformation. Accelerator mass spectrometry quantifies long-lived isotopes such as 14C to sub-attomole sensitivity. We quantified protein interactions with small molecules such as toxins, vitamins, and natural biochemicals at precisions of 1-5%. Micro-proton-induced X-ray emission quantifies elemental abundances in separated metalloprotein samples to nanogram amounts and is capable of quantifying phosphorylated loci in gels. Accelerator-based quantitation is a possible tool for quantifying the genome translation into proteome.
AB - Quantification of specific proteins depends on separation by chromatography or electrophoresis followed by chemical detection schemes such as staining and fluorophore adhesion. Chemical exchange of short-lived isotopes, particularly sulfur, is also prevalent despite the inconveniences of counting radioactivity. Physical methods based on isotopic and elemental analyses offer highly sensitive protein quantitation that has linear response over wide dynamic ranges and is independent of protein conformation. Accelerator mass spectrometry quantifies long-lived isotopes such as 14C to sub-attomole sensitivity. We quantified protein interactions with small molecules such as toxins, vitamins, and natural biochemicals at precisions of 1-5%. Micro-proton-induced X-ray emission quantifies elemental abundances in separated metalloprotein samples to nanogram amounts and is capable of quantifying phosphorylated loci in gels. Accelerator-based quantitation is a possible tool for quantifying the genome translation into proteome.
KW - Accelerator mass spectrometry
KW - Protein
KW - Proteome
KW - Quantitation
KW - Radioisotope
KW - Review
UR - http://www.scopus.com/inward/record.url?scp=0034935772&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034935772&partnerID=8YFLogxK
U2 - 10.1002/1522-2683(200106)22:10<2037::AID-ELPS2037>3.0.CO;2-8
DO - 10.1002/1522-2683(200106)22:10<2037::AID-ELPS2037>3.0.CO;2-8
M3 - Article
C2 - 11465504
AN - SCOPUS:0034935772
VL - 22
SP - 2037
EP - 2045
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 10
ER -