Attenuation of the seizure-induced expression of BDNF mRNA in adult rat brain by an inhibitor of calcium/calmodulin-dependent protein kinases

Karl D Murray, V. Y. Hayes, C. M. Gall, P. J. Isackson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have examined the potential involvement of calcium/calmodulin-dependent protein kinases in the regulation of brain-derived neurotrophic factor mRNA in vivo following kainic acid (kainate)-induced seizure activity by in situ hybridization. KN-62, a specific inhibitor of calcium/calmodulin-dependent protein kinase type II and IV, blocked the characteristic induction of brain-derived neurotrophic factor mRNA seen following seizure activity. This blockade was specific to calcium/calmodulin-dependent protein kinase type II and IV as inhibitors of both protein kinase C and cAMP-dependent protein kinase had no effect. Inhibition of brain-derived neurotrophic factor mRNA increases varied between brain regions; an almost complete inhibition was seen throughout cortical regions, whereas only partial inhibitory effects were noted within hippocampus. A similar inhibition of increased c-fos mRNA was observed throughout cortical, hippocampal and diencephalic regions. The two predominant brain-derived neurotrophic factor transcripts induced by kainate, containing exons I or III, were differentially affected by KN-62. The cortical induction of exon I was blocked by KN-62, whereas exon III was not, providing additional evidence for the differential regulation of individual brain-derived neurotrophic factor transcripts and demonstrating that inhibition of brain-derived neurotrophic factor induction was not due to general blockade of seizure activity throughout the neocortex. These data implicate calcium/calmodulin-dependent protein kinase type II or IV in the regulation of brain-derived neurotrophic factor mRNA in vivo and suggest regionally specific mechanisms occur throughout the brain.

Original languageEnglish (US)
Pages (from-to)377-387
Number of pages11
JournalEuropean Journal of Neuroscience
Volume10
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinases
Brain-Derived Neurotrophic Factor
Calcium-Calmodulin-Dependent Protein Kinase Type 2
KN 62
Seizures
Calcium-Calmodulin-Dependent Protein Kinase Type 4
Messenger RNA
Brain
Kainic Acid
Exons
Neocortex
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C
In Situ Hybridization
Hippocampus

Keywords

  • CamK
  • Exons
  • Kainic acid
  • Kinase
  • Regulation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Attenuation of the seizure-induced expression of BDNF mRNA in adult rat brain by an inhibitor of calcium/calmodulin-dependent protein kinases. / Murray, Karl D; Hayes, V. Y.; Gall, C. M.; Isackson, P. J.

In: European Journal of Neuroscience, Vol. 10, No. 1, 1998, p. 377-387.

Research output: Contribution to journalArticle

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