Attenuated Francisella asiatica iglC mutant induces protective immunity to francisellosis in tilapia

Esteban Soto Martinez, Judy Wiles, Philip Elzer, Kevin Macaluso, John P. Hawke

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Francisella asiatica is a Gram-negative, facultative intracellular bacteria that causes fish francisellosis. Fish francisellosis is a severe sub-acute to chronic granulomatous disease with high mortalities and high infectivity rates in cultured and wild fish. To date, there is no approved vaccine for this widespread emergent disease. The goal of this study was to characterize the efficacy of a defined F. asiatica mutant (ΔiglC) as a live attenuated vaccine against subsequent immersion challenge with the wild-type (WT) organism. In previous work, the ΔiglC was found to be attenuated upon intraperitoneal injection and immersion challenges. In vitro, the ΔiglC exhibited reduced growth in tilapia head-kidney derived macrophages, and was significantly attenuated (p<0.001) as demonstrated by cytopathogenic and apoptosis assays. In this study, the ΔiglC was tested to determine its ability to protect tilapia against challenge with high doses (lethal dose 80) of WT bacteria. Naïve tilapia vaccinated by immersion with a suspension of the ΔiglC and subsequently challenged with WT F. asiatica were protected (90% mean percent survival) from the lethal challenges. F. asiatica-specific antibodies produced in response to immunization with the ΔiglC were subsequently found to protect naïve tilapia against high-dose F. asiatica challenge in passive immunization experiments. Significant protection (p<0.001) was obtained when fish were passively immunized and challenged with 104 and 105CFU/fish of WT F. asiatica; but not when challenged with 106CFU/fish. This is the first report of a defined live attenuated strain providing protection against F. asiatica in fish.

Original languageEnglish (US)
Pages (from-to)593-598
Number of pages6
JournalVaccine
Volume29
Issue number3
DOIs
StatePublished - Jan 10 2011
Externally publishedYes

Fingerprint

Francisella
Tilapia
tilapia (common name)
Immunity
Fishes
immunity
mutants
fish
Immersion
wild fish
live vaccines
immunization
emerging diseases
bacteria
lethal dose
intraperitoneal injection
dosage
Head Kidney
chronic diseases
Bacteria

Keywords

  • Francisella asiatica
  • Immunity
  • Tilapia
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Attenuated Francisella asiatica iglC mutant induces protective immunity to francisellosis in tilapia. / Soto Martinez, Esteban; Wiles, Judy; Elzer, Philip; Macaluso, Kevin; Hawke, John P.

In: Vaccine, Vol. 29, No. 3, 10.01.2011, p. 593-598.

Research output: Contribution to journalArticle

Soto Martinez, Esteban ; Wiles, Judy ; Elzer, Philip ; Macaluso, Kevin ; Hawke, John P. / Attenuated Francisella asiatica iglC mutant induces protective immunity to francisellosis in tilapia. In: Vaccine. 2011 ; Vol. 29, No. 3. pp. 593-598.
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abstract = "Francisella asiatica is a Gram-negative, facultative intracellular bacteria that causes fish francisellosis. Fish francisellosis is a severe sub-acute to chronic granulomatous disease with high mortalities and high infectivity rates in cultured and wild fish. To date, there is no approved vaccine for this widespread emergent disease. The goal of this study was to characterize the efficacy of a defined F. asiatica mutant (ΔiglC) as a live attenuated vaccine against subsequent immersion challenge with the wild-type (WT) organism. In previous work, the ΔiglC was found to be attenuated upon intraperitoneal injection and immersion challenges. In vitro, the ΔiglC exhibited reduced growth in tilapia head-kidney derived macrophages, and was significantly attenuated (p<0.001) as demonstrated by cytopathogenic and apoptosis assays. In this study, the ΔiglC was tested to determine its ability to protect tilapia against challenge with high doses (lethal dose 80) of WT bacteria. Na{\"i}ve tilapia vaccinated by immersion with a suspension of the ΔiglC and subsequently challenged with WT F. asiatica were protected (90{\%} mean percent survival) from the lethal challenges. F. asiatica-specific antibodies produced in response to immunization with the ΔiglC were subsequently found to protect na{\"i}ve tilapia against high-dose F. asiatica challenge in passive immunization experiments. Significant protection (p<0.001) was obtained when fish were passively immunized and challenged with 104 and 105CFU/fish of WT F. asiatica; but not when challenged with 106CFU/fish. This is the first report of a defined live attenuated strain providing protection against F. asiatica in fish.",
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