Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows

R. B. Moeller, Birgit Puschner, R. L. Walker, T. E. Rocke, S. R. Smith, James S Cullor, Alex Ardans

Research output: Contribution to journalArticle

3 Scopus citations


Three adult lactating Holstein cows were injected in the subcutaneous abdominal vein with 175 ng/kg of body weight of Clostridium botulinum type C toxin (451 cow median toxic doses) to determine if this botulinum toxin crosses the blood-milk barrier. Whole blood (in sodium heparin) and clotted blood serum samples were taken at 0 min, 10 min, and 3, 6, 9, and 12 h postinoculation. Milk samples were taken at 0 min and at 3, 6, 9 and 12 h postinoculation. All samples were tested for the presence of the toxin using the mouse bioassay and immunostick ELISA test. The immunostick ELISA identified the toxin in whole blood and the mouse bioassay identified the toxin in serum at all times examined in all 3 animals. Toxin was not identified by either detection method in milk samples collected from the 3 animals. From these results, it appears that Clostridium botulinum type C toxin does not cross from the blood to the milk in detectable concentrations.

Original languageEnglish (US)
Pages (from-to)2529-2533
Number of pages5
JournalJournal of Dairy Science
Issue number6
StatePublished - Jun 2009



  • Botulism
  • Clostridium botulinum toxin type C
  • Milk

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

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