Attachment of fluorescent metal chelates to macromolecules using "bifunctional" chelating agents

Charles S H Leung, Claude F. Meares

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The use of "bifunctional" chelating agents to covalently attach stable chelates of terbium and europium to human serum albumin results in products whose lanthanide fluorescence may be studied easily at micromolar concentrations with standard instrumentation. The lanthanide ions may be added specifically and quantitatively to the protein-bound chelating groups in 0.1 M citrate, pH 6.5. The use of these reagents should greatly reduce ambiguities in the determination of distances between sites on macromolecules by energy transfer measurements.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume75
Issue number1
DOIs
StatePublished - Mar 7 1977

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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