Attaching and effacing E. coli: Microscopic and ultrastructural observations of intestinal infections in pigs

Robert Higgins, G. R. Pearson, C. Wray

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pigs aged from neonate to 8 weeks with naturally occurring diarrhoea were submitted to a Veterinary Investigation Centre for routine diagnosis. They were retrospectively found to be infected with 'attaching and effacing' E. coli (AEEC) on histological and ultrastructural examination of the intestinal tract. Subcultures of E. coli isolated from some of the affected pigs were tested against standard anti-sera and probed for the presence of verotoxin (VT) and 'eae' genes. One strain of E. coli was positive for the VT gene and three VT-negative strains possessed the 'eae' gene. This preliminary report suggests that in naturally occurring diarrhoea of suckling and weaned pigs, AEEC infection can be identified by histological examination of the intestinal mucosa and confirmed by electron microscopy, in the absence of infection by recognised enteric pathogens on microbiological examination.

Original languageEnglish (US)
Pages (from-to)59-62
Number of pages4
JournalAdvances in Experimental Medicine and Biology
Volume412
StatePublished - Jul 4 1997
Externally publishedYes

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Shiga Toxins
Escherichia coli
Swine
Genes
Diarrhea
Infection
Escherichia coli Infections
Intestinal Mucosa
Electron Microscopy
Pathogens
Electron microscopy
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Attaching and effacing E. coli : Microscopic and ultrastructural observations of intestinal infections in pigs. / Higgins, Robert; Pearson, G. R.; Wray, C.

In: Advances in Experimental Medicine and Biology, Vol. 412, 04.07.1997, p. 59-62.

Research output: Contribution to journalArticle

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