Atrial natriuretic peptide modulation of albumin clearance and contrast agent permeability in mouse skeletal muscle and skin: Role in regulation of plasma volume

Fitz Roy E Curry, Cecilie Brekke Rygh, Tine Karlsen, Helge Wiig, Roger H. Adamson, Joyce F. Clark, Yueh Chen Lin, Birgit Gassner, Frits Thorsen, Ingrid Moen, Olav Tenstad, Michaela Kuhn, Rolf K. Reed

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Atrial natriuretic peptide (ANP) via its guanylyl cyclase-A (GC-A) receptor participates in regulation of arterial blood pressure and vascular volume. Previous studies demonstrated that concerted renal diuretic/natriuretic and endothelial permeability effects of ANP cooperate in intravascular volume regulation. We show that the microvascular endothelial contribution to the hypovolaemic action of ANP can be measured by the magnitude of the ANP-induced increase in blood-to-tissue albumin transport, measured as plasma albumin clearance corrected for intravascular volume change, relative to the corresponding increase in ANP-induced renal water excretion. We used a two-tracer method with isotopically labelled albumin to measure clearances in skin and skeletal muscle of: (i) C57BL6 mice; (ii) mice with endothelium-restricted deletion of GC-A (floxed GC-A × tie2-Cre: endothelial cell (EC) GC-A knockout (KO)); and (iii) control littermates (floxed GC-A mice with normal GC-A expression levels). Comparison of albumin clearances in hypervolaemic EC GC-A KO mice with normovolaemic littermates demonstrated that skeletal muscle albumin clearance with ANP treatment accounts for at most 30% of whole body clearance required for ANP to regulate plasma volume. Skin microcirculation responded to ANP similarly. Measurements of permeability to a high molecular mass contrast agent (35 kD Gadomer) by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) enabled repeated measures in individual animals and confirmed small increases in muscle and skin microvascular permeability after ANP. These quantitative methods will enable further evaluation of the contribution of ANP-dependent microvascular beds (such as gastro-intestinal tract) to plasma volume regulation.

Original languageEnglish (US)
Pages (from-to)325-339
Number of pages15
JournalJournal of Physiology
Volume588
Issue number2
DOIs
StatePublished - Jan 2010

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Plasma Volume
Atrial Natriuretic Factor
Contrast Media
Albumins
Permeability
Skeletal Muscle
Skin
Endothelial Cells
Hypovolemia
Capillary Permeability
Microcirculation
atrial natriuretic factor receptor A
Diuretics
Knockout Mice
Serum Albumin
Endothelium
Blood Vessels
Arterial Pressure
Magnetic Resonance Imaging
Kidney

ASJC Scopus subject areas

  • Physiology

Cite this

Atrial natriuretic peptide modulation of albumin clearance and contrast agent permeability in mouse skeletal muscle and skin : Role in regulation of plasma volume. / Curry, Fitz Roy E; Rygh, Cecilie Brekke; Karlsen, Tine; Wiig, Helge; Adamson, Roger H.; Clark, Joyce F.; Lin, Yueh Chen; Gassner, Birgit; Thorsen, Frits; Moen, Ingrid; Tenstad, Olav; Kuhn, Michaela; Reed, Rolf K.

In: Journal of Physiology, Vol. 588, No. 2, 01.2010, p. 325-339.

Research output: Contribution to journalArticle

Curry, FRE, Rygh, CB, Karlsen, T, Wiig, H, Adamson, RH, Clark, JF, Lin, YC, Gassner, B, Thorsen, F, Moen, I, Tenstad, O, Kuhn, M & Reed, RK 2010, 'Atrial natriuretic peptide modulation of albumin clearance and contrast agent permeability in mouse skeletal muscle and skin: Role in regulation of plasma volume', Journal of Physiology, vol. 588, no. 2, pp. 325-339. https://doi.org/10.1113/jphysiol.2009.180463
Curry, Fitz Roy E ; Rygh, Cecilie Brekke ; Karlsen, Tine ; Wiig, Helge ; Adamson, Roger H. ; Clark, Joyce F. ; Lin, Yueh Chen ; Gassner, Birgit ; Thorsen, Frits ; Moen, Ingrid ; Tenstad, Olav ; Kuhn, Michaela ; Reed, Rolf K. / Atrial natriuretic peptide modulation of albumin clearance and contrast agent permeability in mouse skeletal muscle and skin : Role in regulation of plasma volume. In: Journal of Physiology. 2010 ; Vol. 588, No. 2. pp. 325-339.
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abstract = "Atrial natriuretic peptide (ANP) via its guanylyl cyclase-A (GC-A) receptor participates in regulation of arterial blood pressure and vascular volume. Previous studies demonstrated that concerted renal diuretic/natriuretic and endothelial permeability effects of ANP cooperate in intravascular volume regulation. We show that the microvascular endothelial contribution to the hypovolaemic action of ANP can be measured by the magnitude of the ANP-induced increase in blood-to-tissue albumin transport, measured as plasma albumin clearance corrected for intravascular volume change, relative to the corresponding increase in ANP-induced renal water excretion. We used a two-tracer method with isotopically labelled albumin to measure clearances in skin and skeletal muscle of: (i) C57BL6 mice; (ii) mice with endothelium-restricted deletion of GC-A (floxed GC-A × tie2-Cre: endothelial cell (EC) GC-A knockout (KO)); and (iii) control littermates (floxed GC-A mice with normal GC-A expression levels). Comparison of albumin clearances in hypervolaemic EC GC-A KO mice with normovolaemic littermates demonstrated that skeletal muscle albumin clearance with ANP treatment accounts for at most 30{\%} of whole body clearance required for ANP to regulate plasma volume. Skin microcirculation responded to ANP similarly. Measurements of permeability to a high molecular mass contrast agent (35 kD Gadomer) by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) enabled repeated measures in individual animals and confirmed small increases in muscle and skin microvascular permeability after ANP. These quantitative methods will enable further evaluation of the contribution of ANP-dependent microvascular beds (such as gastro-intestinal tract) to plasma volume regulation.",
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AU - Lin, Yueh Chen

AU - Gassner, Birgit

AU - Thorsen, Frits

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AU - Reed, Rolf K.

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