Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force

Vasilios A. Morikis, Chris Radecke, Yanyan Jiang, Volkmar Heinrich, Fitz Roy Curry, Scott I. Simon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BACKGROUND: Recombinant atrial natriuretic peptide (ANP) is administered in patients with acute heart failure in Japan to improve renal function and hemodynamics, but its anti-inflammatory effect on activated leukocytes may also contribute to its therapeutic efficacy.

OBJECTIVE: Examine unconventional role of ANP in neutrophil adhesion to inflamed endothelium.

METHODS: Human neutrophils were perfused over endothelial monolayers in a microfluidic lab-chip assay. Cell rheology was assessed by micropipette aspiration to assess changes in cortical tension and viscosity. Fluorescence microscopy was applied to measure adhesive contact area and β2-integrin focal bond formation.

RESULTS: ANP inhibited neutrophil rolling and firm adhesion without influencing the upregulation of cellular adhesion molecules on endothelium or the regulation of high affinity CD18 and shedding of L-selectin during neutrophil activation. Exposed to fluid shear, integrin mediated arrest was disrupted with ANP treatment, which elicited formation of long tethers and diminished cell spreading and contact. This correlated with a ∼40% increase in neutrophil viscosity and a reduction in the adhesive footprint.

CONCLUSIONS: A decrease in cell deformation and neutrophil flattening with ANP results in fewer integrin bond clusters, which translates to higher tensile forces and impaired adhesion strengthening and cell detachment.

Original languageEnglish (US)
Pages (from-to)447-463
Number of pages17
JournalBiorheology
Volume52
Issue number5-6
DOIs
StatePublished - 2015

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Neutrophil Infiltration
Atrial Natriuretic Factor
Endothelium
Neutrophils
Down-Regulation
Integrins
Viscosity
Adhesives
L-Selectin
Neutrophil Activation
Microfluidics
Rheology
Fluorescence Microscopy
Cell Adhesion
Japan
Leukocytes
Anti-Inflammatory Agents
Up-Regulation
Heart Failure
Hemodynamics

Keywords

  • adhesion
  • Atrial natriuretic peptide
  • inflammation
  • neutrophil

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force. / Morikis, Vasilios A.; Radecke, Chris; Jiang, Yanyan; Heinrich, Volkmar; Curry, Fitz Roy; Simon, Scott I.

In: Biorheology, Vol. 52, No. 5-6, 2015, p. 447-463.

Research output: Contribution to journalArticle

Morikis, Vasilios A. ; Radecke, Chris ; Jiang, Yanyan ; Heinrich, Volkmar ; Curry, Fitz Roy ; Simon, Scott I. / Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force. In: Biorheology. 2015 ; Vol. 52, No. 5-6. pp. 447-463.
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AU - Curry, Fitz Roy

AU - Simon, Scott I.

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AB - BACKGROUND: Recombinant atrial natriuretic peptide (ANP) is administered in patients with acute heart failure in Japan to improve renal function and hemodynamics, but its anti-inflammatory effect on activated leukocytes may also contribute to its therapeutic efficacy.OBJECTIVE: Examine unconventional role of ANP in neutrophil adhesion to inflamed endothelium.METHODS: Human neutrophils were perfused over endothelial monolayers in a microfluidic lab-chip assay. Cell rheology was assessed by micropipette aspiration to assess changes in cortical tension and viscosity. Fluorescence microscopy was applied to measure adhesive contact area and β2-integrin focal bond formation.RESULTS: ANP inhibited neutrophil rolling and firm adhesion without influencing the upregulation of cellular adhesion molecules on endothelium or the regulation of high affinity CD18 and shedding of L-selectin during neutrophil activation. Exposed to fluid shear, integrin mediated arrest was disrupted with ANP treatment, which elicited formation of long tethers and diminished cell spreading and contact. This correlated with a ∼40% increase in neutrophil viscosity and a reduction in the adhesive footprint.CONCLUSIONS: A decrease in cell deformation and neutrophil flattening with ANP results in fewer integrin bond clusters, which translates to higher tensile forces and impaired adhesion strengthening and cell detachment.

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