ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus

Eissa Hanna, Ian J. MacRae, Daniel C. Medina, Andrew J Fisher, Irwin H. Segel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus is a bacterial ortholog of the enzyme from filamentous fungi. (The subunit contains an adenosine 5′-phosphosulfate (APS) kinase-like, C-terminal domain.) The enzyme is highly heat stable with a half-life > 1 h at 90°C. Steady-state kinetics are consistent with a random A-B, ordered P-Q mechanism where A = MgATP, B = SO2- 4, P = PPi, and Q = APS. The kinetic constants suggest that the enzyme is optimized to act in the direction of ATP + sulfate formation. Chlorate is competitive with sulfate and with APS. In sulfur chemolithotrophs, ATP sulfurylase provides an efficient route for recycling PPi produced by biosynthetic reactions. However, the protein possesses low APS kinase activity. Consequently, it may also function to produce PAPS for sulfate ester formation or sulfate assimilation when hydrogen serves as the energy source and a reduced inorganic sulfur source is unavailable.

Original languageEnglish (US)
Pages (from-to)275-288
Number of pages14
JournalArchives of Biochemistry and Biophysics
Volume406
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Sulfate Adenylyltransferase
adenylylsulfate kinase
Sulfates
Adenosine Phosphosulfate
Sulfur
Enzymes
Adenosine Triphosphate
Chlorates
Kinetics
Recycling
Fungi
Half-Life
Hydrogen
Esters
Hot Temperature
Proteins

Keywords

  • Adenylylsulfate (APS) kinase, from Aquifex aeolicus, hyperthermophile, ATP sulfurylase from
  • ATP sulfurylase, from Aquifex aeolicus
  • Chemolithotroph, ATP sulfurylase, and APS kinase in
  • Sulfurylase, from a hyperthermophilic bacterium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus. / Hanna, Eissa; MacRae, Ian J.; Medina, Daniel C.; Fisher, Andrew J; Segel, Irwin H.

In: Archives of Biochemistry and Biophysics, Vol. 406, No. 2, 2002, p. 275-288.

Research output: Contribution to journalArticle

Hanna, Eissa ; MacRae, Ian J. ; Medina, Daniel C. ; Fisher, Andrew J ; Segel, Irwin H. / ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus. In: Archives of Biochemistry and Biophysics. 2002 ; Vol. 406, No. 2. pp. 275-288.
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