Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure

Susanna L. Lundström, Bettina Levänen, Malin Nording, Anna Klepczynska-Nyström, Magnus Sköld, Jesper Z. Haeggström, Johan Grunewald, Magnus Svartengren, Bruce D. Hammock, Britt Marie Larsson, Anders Eklund, Åsa M. Wheelock, Craig E. Wheelock

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Asthma is a chronic inflammatory lung disease that causes significant morbidity and mortality worldwide. Air pollutants such as particulate matter (PM) and oxidants are important factors in causing exacerbations in asthmatics, and the source and composition of pollutants greatly affects pathological implications. Objectives: This randomized crossover study investigated responses of the respiratory system to Stockholm subway air in asthmatics and healthy individuals. Eicosanoids and other oxylipins were quantified in the distal lung to provide a measure of shifts in lipid mediators in association with exposure to subway air relative to ambient air. Methods: Sixty-four oxylipins representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened using liquid chromatography-tandem mass spectrometry (LC-MS/MS) of bronchoalveolar lavage (BAL)-fluid. Validations through immunocytochemistry staining of BAL-cells were performed for 15-LOX-1, COX-1, COX-2 and peroxisome proliferator-activated receptor gamma (PPARγ). Multivariate statistics were employed to interrogate acquired oxylipin and immunocytochemistry data in combination with patient clinical information. Results: Asthmatics and healthy individuals exhibited divergent oxylipin profiles following exposure to ambient and subway air. Significant changes were observed in 8 metabolites of linoleic- and α-linolenic acid synthesized via the 15-LOX pathway, and of the COX product prostaglandin E 2 (PGE 2). Oxylipin levels were increased in healthy individuals following exposure to subway air, whereas asthmatics evidenced decreases or no change. Conclusions: Several of the altered oxylipins have known or suspected bronchoprotective or anti-inflammatory effects, suggesting a possible reduced anti-inflammatory response in asthmatics following exposure to subway air. These observations may have ramifications for sensitive subpopulations in urban areas.

Original languageEnglish (US)
Article numbere23864
JournalPLoS One
Volume6
Issue number8
DOIs
StatePublished - Aug 29 2011

Fingerprint

Oxylipins
Railroads
oxylipins
Subways
Air
air
prostaglandin synthase
Arachidonate 15-Lipoxygenase
lipoxygenase
Lipoxygenase
Prostaglandin-Endoperoxide Synthases
immunocytochemistry
Anti-Inflammatory Agents
Immunohistochemistry
exposure profile
Respiratory system
pollutants
Air Pollutants
Cyclooxygenase 1
Pulmonary diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lundström, S. L., Levänen, B., Nording, M., Klepczynska-Nyström, A., Sköld, M., Haeggström, J. Z., ... Wheelock, C. E. (2011). Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure. PLoS One, 6(8), [e23864]. https://doi.org/10.1371/journal.pone.0023864

Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure. / Lundström, Susanna L.; Levänen, Bettina; Nording, Malin; Klepczynska-Nyström, Anna; Sköld, Magnus; Haeggström, Jesper Z.; Grunewald, Johan; Svartengren, Magnus; Hammock, Bruce D.; Larsson, Britt Marie; Eklund, Anders; Wheelock, Åsa M.; Wheelock, Craig E.

In: PLoS One, Vol. 6, No. 8, e23864, 29.08.2011.

Research output: Contribution to journalArticle

Lundström, SL, Levänen, B, Nording, M, Klepczynska-Nyström, A, Sköld, M, Haeggström, JZ, Grunewald, J, Svartengren, M, Hammock, BD, Larsson, BM, Eklund, A, Wheelock, ÅM & Wheelock, CE 2011, 'Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure', PLoS One, vol. 6, no. 8, e23864. https://doi.org/10.1371/journal.pone.0023864
Lundström SL, Levänen B, Nording M, Klepczynska-Nyström A, Sköld M, Haeggström JZ et al. Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure. PLoS One. 2011 Aug 29;6(8). e23864. https://doi.org/10.1371/journal.pone.0023864
Lundström, Susanna L. ; Levänen, Bettina ; Nording, Malin ; Klepczynska-Nyström, Anna ; Sköld, Magnus ; Haeggström, Jesper Z. ; Grunewald, Johan ; Svartengren, Magnus ; Hammock, Bruce D. ; Larsson, Britt Marie ; Eklund, Anders ; Wheelock, Åsa M. ; Wheelock, Craig E. / Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure. In: PLoS One. 2011 ; Vol. 6, No. 8.
@article{7772bbde5b134e48ad12d77a0bf1550a,
title = "Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure",
abstract = "Background: Asthma is a chronic inflammatory lung disease that causes significant morbidity and mortality worldwide. Air pollutants such as particulate matter (PM) and oxidants are important factors in causing exacerbations in asthmatics, and the source and composition of pollutants greatly affects pathological implications. Objectives: This randomized crossover study investigated responses of the respiratory system to Stockholm subway air in asthmatics and healthy individuals. Eicosanoids and other oxylipins were quantified in the distal lung to provide a measure of shifts in lipid mediators in association with exposure to subway air relative to ambient air. Methods: Sixty-four oxylipins representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened using liquid chromatography-tandem mass spectrometry (LC-MS/MS) of bronchoalveolar lavage (BAL)-fluid. Validations through immunocytochemistry staining of BAL-cells were performed for 15-LOX-1, COX-1, COX-2 and peroxisome proliferator-activated receptor gamma (PPARγ). Multivariate statistics were employed to interrogate acquired oxylipin and immunocytochemistry data in combination with patient clinical information. Results: Asthmatics and healthy individuals exhibited divergent oxylipin profiles following exposure to ambient and subway air. Significant changes were observed in 8 metabolites of linoleic- and α-linolenic acid synthesized via the 15-LOX pathway, and of the COX product prostaglandin E 2 (PGE 2). Oxylipin levels were increased in healthy individuals following exposure to subway air, whereas asthmatics evidenced decreases or no change. Conclusions: Several of the altered oxylipins have known or suspected bronchoprotective or anti-inflammatory effects, suggesting a possible reduced anti-inflammatory response in asthmatics following exposure to subway air. These observations may have ramifications for sensitive subpopulations in urban areas.",
author = "Lundstr{\"o}m, {Susanna L.} and Bettina Lev{\"a}nen and Malin Nording and Anna Klepczynska-Nystr{\"o}m and Magnus Sk{\"o}ld and Haeggstr{\"o}m, {Jesper Z.} and Johan Grunewald and Magnus Svartengren and Hammock, {Bruce D.} and Larsson, {Britt Marie} and Anders Eklund and Wheelock, {{\AA}sa M.} and Wheelock, {Craig E.}",
year = "2011",
month = "8",
day = "29",
doi = "10.1371/journal.pone.0023864",
language = "English (US)",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure

AU - Lundström, Susanna L.

AU - Levänen, Bettina

AU - Nording, Malin

AU - Klepczynska-Nyström, Anna

AU - Sköld, Magnus

AU - Haeggström, Jesper Z.

AU - Grunewald, Johan

AU - Svartengren, Magnus

AU - Hammock, Bruce D.

AU - Larsson, Britt Marie

AU - Eklund, Anders

AU - Wheelock, Åsa M.

AU - Wheelock, Craig E.

PY - 2011/8/29

Y1 - 2011/8/29

N2 - Background: Asthma is a chronic inflammatory lung disease that causes significant morbidity and mortality worldwide. Air pollutants such as particulate matter (PM) and oxidants are important factors in causing exacerbations in asthmatics, and the source and composition of pollutants greatly affects pathological implications. Objectives: This randomized crossover study investigated responses of the respiratory system to Stockholm subway air in asthmatics and healthy individuals. Eicosanoids and other oxylipins were quantified in the distal lung to provide a measure of shifts in lipid mediators in association with exposure to subway air relative to ambient air. Methods: Sixty-four oxylipins representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened using liquid chromatography-tandem mass spectrometry (LC-MS/MS) of bronchoalveolar lavage (BAL)-fluid. Validations through immunocytochemistry staining of BAL-cells were performed for 15-LOX-1, COX-1, COX-2 and peroxisome proliferator-activated receptor gamma (PPARγ). Multivariate statistics were employed to interrogate acquired oxylipin and immunocytochemistry data in combination with patient clinical information. Results: Asthmatics and healthy individuals exhibited divergent oxylipin profiles following exposure to ambient and subway air. Significant changes were observed in 8 metabolites of linoleic- and α-linolenic acid synthesized via the 15-LOX pathway, and of the COX product prostaglandin E 2 (PGE 2). Oxylipin levels were increased in healthy individuals following exposure to subway air, whereas asthmatics evidenced decreases or no change. Conclusions: Several of the altered oxylipins have known or suspected bronchoprotective or anti-inflammatory effects, suggesting a possible reduced anti-inflammatory response in asthmatics following exposure to subway air. These observations may have ramifications for sensitive subpopulations in urban areas.

AB - Background: Asthma is a chronic inflammatory lung disease that causes significant morbidity and mortality worldwide. Air pollutants such as particulate matter (PM) and oxidants are important factors in causing exacerbations in asthmatics, and the source and composition of pollutants greatly affects pathological implications. Objectives: This randomized crossover study investigated responses of the respiratory system to Stockholm subway air in asthmatics and healthy individuals. Eicosanoids and other oxylipins were quantified in the distal lung to provide a measure of shifts in lipid mediators in association with exposure to subway air relative to ambient air. Methods: Sixty-four oxylipins representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened using liquid chromatography-tandem mass spectrometry (LC-MS/MS) of bronchoalveolar lavage (BAL)-fluid. Validations through immunocytochemistry staining of BAL-cells were performed for 15-LOX-1, COX-1, COX-2 and peroxisome proliferator-activated receptor gamma (PPARγ). Multivariate statistics were employed to interrogate acquired oxylipin and immunocytochemistry data in combination with patient clinical information. Results: Asthmatics and healthy individuals exhibited divergent oxylipin profiles following exposure to ambient and subway air. Significant changes were observed in 8 metabolites of linoleic- and α-linolenic acid synthesized via the 15-LOX pathway, and of the COX product prostaglandin E 2 (PGE 2). Oxylipin levels were increased in healthy individuals following exposure to subway air, whereas asthmatics evidenced decreases or no change. Conclusions: Several of the altered oxylipins have known or suspected bronchoprotective or anti-inflammatory effects, suggesting a possible reduced anti-inflammatory response in asthmatics following exposure to subway air. These observations may have ramifications for sensitive subpopulations in urban areas.

UR - http://www.scopus.com/inward/record.url?scp=80052302764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052302764&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0023864

DO - 10.1371/journal.pone.0023864

M3 - Article

C2 - 21897859

AN - SCOPUS:80052302764

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e23864

ER -