Association of the tensin N-terminal protein-tyrosine phosphatase domain with the α isoform of protein phosphatase-1 in focal adhesions

Masumi Eto, Jason Kirkbride, Elizabeth Elliott, Su Hao Lo, David L. Brautigan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Focal adhesions attach cultured cells to the extracellular matrix, and we found endogenous protein phosphatase-1α isoform (PP1α) localized in adhesions across the entire area of adherent fibroblasts. However, in fibroblasts migrating into a scrape wound or spreading after replating PP1α did not appear in adhesions near the leading edge but was recruited into other adhesions coincident in time and space with incorporation of tensin. Endogenous tensin and PP1α co-precipitated from cell lysates with isoform-specific PP1 antibodies. Chemical cross-linking of focal adhesion preparations with Lomant's reagent demonstrated molecular proximity of endogenous PP1α and tensin, whereas neither focal adhesion kinase nor vinculin was cross-linked and co-precipitated with PP1α, suggesting distinct spatial subdomains within adhesions. Transient expression of truncated tensin showed the N-terminal 360 residues, which comprise a protein-tyrosine phosphatase domain, alone were sufficient for isoform-selective co-precipitation of co-expressed PP1α. Human prostate cancer PC3 cells are deficient in tensin relative to fibroblasts and have fewer, mostly peripheral adhesions. Transient expression of green fluorescent protein tensin in these cancer cells induced formation of adhesions and recruited endogenous PP1α into those adhesions. Thus, the protein-tyrosine phosphatase domain of tensin exhibits isoform-specific association with PP1α in a restricted spatial region of adhesions that are formed during cell migration.

Original languageEnglish (US)
Pages (from-to)17806-17815
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number24
DOIs
StatePublished - Jun 15 2007

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Protein Phosphatase 1
Protein Tyrosine Phosphatases
Focal Adhesions
Protein Isoforms
Adhesion
Fibroblasts
Tensins
Cells
Vinculin
Focal Adhesion Protein-Tyrosine Kinases
Coprecipitation
Green Fluorescent Proteins
Cell Movement
Extracellular Matrix
Cultured Cells
Prostatic Neoplasms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Association of the tensin N-terminal protein-tyrosine phosphatase domain with the α isoform of protein phosphatase-1 in focal adhesions. / Eto, Masumi; Kirkbride, Jason; Elliott, Elizabeth; Lo, Su Hao; Brautigan, David L.

In: Journal of Biological Chemistry, Vol. 282, No. 24, 15.06.2007, p. 17806-17815.

Research output: Contribution to journalArticle

Eto, Masumi ; Kirkbride, Jason ; Elliott, Elizabeth ; Lo, Su Hao ; Brautigan, David L. / Association of the tensin N-terminal protein-tyrosine phosphatase domain with the α isoform of protein phosphatase-1 in focal adhesions. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 24. pp. 17806-17815.
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