Association of plasma IgM with body size, histopathologic changes, and plasma chemistries in adult Pacific herring Clupea pallasi

Corrine R. Davis, Gary D. Marty, Mark A. Adkison, Ellen F. Freiberg, Ronald Hedrick

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Pacific herring Clupea pallasi immunoglobulin is an IgM-like molecule comprised of heavy and light chains with molecular weights of 79 and 25 to 27 kD, respectively. Purified immunoglobulin was used to generate highly specific polyclonal antibodies for development of a sandwich ELISA. The ELISA was used to quantify total plasma IgM in 602 Pacific herring captured in Prince William Sound and Sitka Sound, Alaska, USA. Plasma IgM concentrations ranged from 0.13 to 5.32 mg ml-1. Using multiple stepwise regression analysis, plasma IgM was highly correlated (p ≤ 0.01) with body length, Ichthyophonus hoferi infection plasma albumin, plasma cholesterol, liver macrophage aggregates, and focal skin reddening. I. hoferi was the only organism significantly associated with plasma IgM, Gender, site, and season (spring vs fall) did not contribute to significant differences in plasma IgM. This study contributes to the understanding of the interaction of body size, plasma chemistries, and pathological changes upon circulating immunoglobulins in fish.

Original languageEnglish (US)
Pages (from-to)125-133
Number of pages9
JournalDiseases of Aquatic Organisms
Volume38
Issue number2
DOIs
StatePublished - Nov 8 1999

Fingerprint

Clupea
blood chemistry
immunoglobulins
body size
plasma
Ichthyophonus
enzyme-linked immunosorbent assay
protein aggregates
sandwiches
polyclonal antibodies
skin (animal)
body length
albumins
macrophages
regression analysis
cholesterol
molecular weight
liver
gender
organisms

Keywords

  • Body length
  • Clupea pallasi
  • ELISA
  • Euglobulin
  • Fish
  • Histopathology
  • Ichthyophonus hoferi
  • IgM
  • Immunoglobulin M
  • Pacific herring
  • Plasma chemistry

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Association of plasma IgM with body size, histopathologic changes, and plasma chemistries in adult Pacific herring Clupea pallasi. / Davis, Corrine R.; Marty, Gary D.; Adkison, Mark A.; Freiberg, Ellen F.; Hedrick, Ronald.

In: Diseases of Aquatic Organisms, Vol. 38, No. 2, 08.11.1999, p. 125-133.

Research output: Contribution to journalArticle

Davis, Corrine R. ; Marty, Gary D. ; Adkison, Mark A. ; Freiberg, Ellen F. ; Hedrick, Ronald. / Association of plasma IgM with body size, histopathologic changes, and plasma chemistries in adult Pacific herring Clupea pallasi. In: Diseases of Aquatic Organisms. 1999 ; Vol. 38, No. 2. pp. 125-133.
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