Association of Obesity with Serum Leptin, Adiponectin, and Serotonin and Gut Microflora in Beagle Dogs

H. J. Park, S. E. Lee, H. B. Kim, R. E. Isaacson, Kyoung Won Seo, K. H. Song

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Serotonin (5-hydroxytryptamine, 5HT) is involved in hypothalamic regulation of energy consumption. Also, the gut microbiome can influence neuronal signaling to the brain through vagal afferent neurons. Therefore, serotonin concentrations in the central nervous system and the composition of the microbiota can be related to obesity. Objective: To examine adipokine, and, serotonin concentrations, and the gut microbiota in lean dogs and dogs with experimentally induced obesity. Animals: Fourteen healthy Beagle dogs were used in this study. Methods: Seven Beagle dogs in the obese group were fed commercial food ad libitum, over a period of 6 months to increase their weight and seven Beagle dogs in lean group were fed a restricted amount of the same diet to maintain optimal body condition over a period of 6 months. Peripheral leptin, adiponectin, 5HT, and cerebrospinal fluid (CSF-5HT) levels were measured by ELISA. Fecal samples were collected in lean and obese groups 6 months after obesity was induced. Targeted pyrosequencing of the 16S rRNA gene was performed using a Genome Sequencer FLX plus system. Results: Leptin concentrations were higher in the obese group (1.98 ± 1.00) compared to those of the lean group (1.12 ± 0.07, P = .025). Adiponectin and 5-hydroytryptamine of cerebrospinal fluid (CSF-5HT) concentrations were higher in the lean group (27.1 ± 7.28) than in the obese group (14.4 ± 5.40, P = .018). Analysis of the microbiome revealed that the diversity of the microbial community was lower in the obese group. Microbes from the phylum Firmicutes (85%) were predominant group in the gut microbiota of lean dogs. However, bacteria from the phylum Proteobacteria (76%) were the predominant group in the gut microbiota of dogs in the obese group. Conclusions and Clinical Importance: Decreased 5HT levels in obese group might increase the risk of obesity because of increased appetite. Microflora enriched with gram-negative might be related with chronic inflammation status in obese dogs.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalJournal of Veterinary Internal Medicine
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

adiponectin
Adiponectin
Leptin
Beagle
leptin
intestinal microorganisms
serotonin
Serotonin
obesity
Obesity
Dogs
dogs
Serum
Microbiota
cerebrospinal fluid
Cerebrospinal Fluid
hypothalamic regulation
microorganisms
adipokines
Afferent Neurons

Keywords

  • Adipokine
  • Dog
  • Microbiome
  • Microbiota
  • Nutrition
  • Obesity
  • Pyrosequencing
  • Serotonin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Association of Obesity with Serum Leptin, Adiponectin, and Serotonin and Gut Microflora in Beagle Dogs. / Park, H. J.; Lee, S. E.; Kim, H. B.; Isaacson, R. E.; Seo, Kyoung Won; Song, K. H.

In: Journal of Veterinary Internal Medicine, Vol. 29, No. 1, 01.01.2015, p. 43-50.

Research output: Contribution to journalArticle

Park, H. J. ; Lee, S. E. ; Kim, H. B. ; Isaacson, R. E. ; Seo, Kyoung Won ; Song, K. H. / Association of Obesity with Serum Leptin, Adiponectin, and Serotonin and Gut Microflora in Beagle Dogs. In: Journal of Veterinary Internal Medicine. 2015 ; Vol. 29, No. 1. pp. 43-50.
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AU - Seo, Kyoung Won

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