Association of minimum inhibitory concentration cluster patterns with dairy management practices for environmental bacteria isolated from bulk tank milk

J. H. Kirk, Brenda Mccowan, Edward R Atwill, K. S. Glenn, G. E. Higginbotham, C. A. Collar, A. Castillo, B. A. Reed, N. G. Peterson, James S Cullor

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Environmental bacteria have emerged over the past few years to become significant causes of mastitis. Bacteria in this group are often reported by practicing veterinarians to be increasingly resistant to intramammary therapy and responsible for elevated bulk tank somatic cell counts. The purpose of this study was to determine the extent of association of the minimum inhibitory concentrations for selected antimicrobial agents with environmental bacteria isolated from bulk tank milk on California dairies and their housing facilities, husbandry practices, and antimicrobic-use strategies. Bulk tank milk samples were collected from 2 dairy cooperatives that had their milk cultured at the Milk Quality Laboratory, University of California Davis, Veterinary Medicine Teaching and Research Center in Tulare, CA. Samples were collected from July 2001 through March 2002 on 88 d; and 404 environmental bacteria isolated from 93 dairies were found. Minimum inhibitory concentrations were determined on 337 of the isolates for 10 antimicrobial agents. Cluster analysis was performed on the minimum inhibitory concentration values for each organism, and 4 antimicrobial clusters with varying degrees of resistance were found. A 69-question survey questionnaire was completed on-farm for 49 of the 73 dairies that had at least 3 environmental bacterial isolates. The questionnaire sought information on housing facilities, milking management, mastitis prevention, antimicrobial usage strategies, and owner/veterinary involvement in disease control and prevention. Multinomial logistic regression analysis found significant associations between the antimicrobial agent-resistance cluster groups and some of the housing and bedding practices, failure to dry udders before milking, and antimicrobial treatment of nonmastitis conditions. No association was noted for antimicrobial agent treatment of mastitis and the resistance cluster patterns.

Original languageEnglish (US)
Pages (from-to)3710-3720
Number of pages11
JournalJournal of Dairy Science
Volume88
Issue number10
StatePublished - Oct 2005

Fingerprint

milk tanks
Practice Management
Microbial Sensitivity Tests
minimum inhibitory concentration
Anti-Infective Agents
Mastitis
dairies
Milk
anti-infective agents
Bacteria
milk
bacteria
mastitis
Animal Mammary Glands
Veterinary Medicine
Veterinarians
milking
questionnaires
Cluster Analysis
dairy cooperatives

Keywords

  • Bulk tank milk
  • Cluster pattern
  • Environmental bacteria
  • Minimum inhibitory concentration

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Food Science

Cite this

Association of minimum inhibitory concentration cluster patterns with dairy management practices for environmental bacteria isolated from bulk tank milk. / Kirk, J. H.; Mccowan, Brenda; Atwill, Edward R; Glenn, K. S.; Higginbotham, G. E.; Collar, C. A.; Castillo, A.; Reed, B. A.; Peterson, N. G.; Cullor, James S.

In: Journal of Dairy Science, Vol. 88, No. 10, 10.2005, p. 3710-3720.

Research output: Contribution to journalArticle

Kirk, J. H. ; Mccowan, Brenda ; Atwill, Edward R ; Glenn, K. S. ; Higginbotham, G. E. ; Collar, C. A. ; Castillo, A. ; Reed, B. A. ; Peterson, N. G. ; Cullor, James S. / Association of minimum inhibitory concentration cluster patterns with dairy management practices for environmental bacteria isolated from bulk tank milk. In: Journal of Dairy Science. 2005 ; Vol. 88, No. 10. pp. 3710-3720.
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