Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients

Yu Yuan Li, Quan Xing Ge, Jie Cao, Yong Jian Zhou, Yan Lei Du, Bo Shen, Yu-Jui Yvonne Wan, Yu Qiang Nie

Research output: Contribution to journalArticle

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Abstract

AIM: To investigate Fusobacterium nucleatum (F. nucleatum ) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS: The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results. RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1%) CRC samples. The abundance of F. nucleatum determined by 2-ΔCT was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1%)] than in the under-abundance group [0/13 (0%)] (P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25th 3, 75th 10 vs 2, 25th 1, 75th 5) (P < 0.01). CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.

Original languageEnglish (US)
Pages (from-to)3227-3233
Number of pages7
JournalWorld Journal of Gastroenterology
Volume22
Issue number11
DOIs
StatePublished - Mar 21 2016

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Fusobacterium Infections
Fusobacterium nucleatum
Colorectal Neoplasms
Fluorescence In Situ Hybridization
Polymerase Chain Reaction
Neoplasms

Keywords

  • Colorectal cancer
  • Fluorescence in situ hybridization
  • Fluorescent quantitative polymerase chain reaction
  • Fusobacterium nucleatum
  • Metastases

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Li, Y. Y., Ge, Q. X., Cao, J., Zhou, Y. J., Du, Y. L., Shen, B., ... Nie, Y. Q. (2016). Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients. World Journal of Gastroenterology, 22(11), 3227-3233. https://doi.org/10.3748/wjg.v22.i11.3227

Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients. / Li, Yu Yuan; Ge, Quan Xing; Cao, Jie; Zhou, Yong Jian; Du, Yan Lei; Shen, Bo; Wan, Yu-Jui Yvonne; Nie, Yu Qiang.

In: World Journal of Gastroenterology, Vol. 22, No. 11, 21.03.2016, p. 3227-3233.

Research output: Contribution to journalArticle

Li, Yu Yuan ; Ge, Quan Xing ; Cao, Jie ; Zhou, Yong Jian ; Du, Yan Lei ; Shen, Bo ; Wan, Yu-Jui Yvonne ; Nie, Yu Qiang. / Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients. In: World Journal of Gastroenterology. 2016 ; Vol. 22, No. 11. pp. 3227-3233.
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abstract = "AIM: To investigate Fusobacterium nucleatum (F. nucleatum ) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS: The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results. RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1{\%}) CRC samples. The abundance of F. nucleatum determined by 2-ΔCT was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1{\%})] than in the under-abundance group [0/13 (0{\%})] (P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25th 3, 75th 10 vs 2, 25th 1, 75th 5) (P < 0.01). CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.",
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AU - Li, Yu Yuan

AU - Ge, Quan Xing

AU - Cao, Jie

AU - Zhou, Yong Jian

AU - Du, Yan Lei

AU - Shen, Bo

AU - Wan, Yu-Jui Yvonne

AU - Nie, Yu Qiang

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N2 - AIM: To investigate Fusobacterium nucleatum (F. nucleatum ) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS: The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results. RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1%) CRC samples. The abundance of F. nucleatum determined by 2-ΔCT was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1%)] than in the under-abundance group [0/13 (0%)] (P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25th 3, 75th 10 vs 2, 25th 1, 75th 5) (P < 0.01). CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.

AB - AIM: To investigate Fusobacterium nucleatum (F. nucleatum ) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS: The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results. RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1%) CRC samples. The abundance of F. nucleatum determined by 2-ΔCT was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1%)] than in the under-abundance group [0/13 (0%)] (P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25th 3, 75th 10 vs 2, 25th 1, 75th 5) (P < 0.01). CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.

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KW - Fluorescence in situ hybridization

KW - Fluorescent quantitative polymerase chain reaction

KW - Fusobacterium nucleatum

KW - Metastases

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