Association of artery wall hypoxia and cellular proliferation at a vascular anastomosis

Eugene S Lee, G. Eric Bauer, Michael P. Caldwell, Steven M. Santilli

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalJournal of Surgical Research
Volume91
Issue number1
DOIs
StatePublished - Jun 1 2000
Externally publishedYes

Fingerprint

Blood Vessels
Arteries
Cell Proliferation
Tunica Intima
Bromodeoxyuridine
Rabbits
Transplants
Staining and Labeling
Hyperplasia
Aorta
Oxygen
Polytetrafluoroethylene
Microelectrodes
Ambulatory Surgical Procedures
Hypoxia
Students

Keywords

  • Artery
  • Hypoxia
  • Intimal hyperplasia
  • Smooth muscle cells

ASJC Scopus subject areas

  • Surgery

Cite this

Association of artery wall hypoxia and cellular proliferation at a vascular anastomosis. / Lee, Eugene S; Bauer, G. Eric; Caldwell, Michael P.; Santilli, Steven M.

In: Journal of Surgical Research, Vol. 91, No. 1, 01.06.2000, p. 32-37.

Research output: Contribution to journalArticle

Lee, Eugene S ; Bauer, G. Eric ; Caldwell, Michael P. ; Santilli, Steven M. / Association of artery wall hypoxia and cellular proliferation at a vascular anastomosis. In: Journal of Surgical Research. 2000 ; Vol. 91, No. 1. pp. 32-37.
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abstract = "Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3{\%}, versus BrDU-staining ratio, 1.4 ± 1{\%}, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.",
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N2 - Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.

AB - Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.

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