Abstract
Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 32-37 |
| Number of pages | 6 |
| Journal | Journal of Surgical Research |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1 2000 |
| Externally published | Yes |
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Keywords
- Artery
- Hypoxia
- Intimal hyperplasia
- Smooth muscle cells
ASJC Scopus subject areas
- Surgery
Cite this
Association of artery wall hypoxia and cellular proliferation at a vascular anastomosis. / Lee, Eugene S; Bauer, G. Eric; Caldwell, Michael P.; Santilli, Steven M.
In: Journal of Surgical Research, Vol. 91, No. 1, 01.06.2000, p. 32-37.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of artery wall hypoxia and cellular proliferation at a vascular anastomosis
AU - Lee, Eugene S
AU - Bauer, G. Eric
AU - Caldwell, Michael P.
AU - Santilli, Steven M.
PY - 2000/6/1
Y1 - 2000/6/1
N2 - Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.
AB - Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 29, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2'- deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counter-staining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize. (C) 2000 Academic Press.
KW - Artery
KW - Hypoxia
KW - Intimal hyperplasia
KW - Smooth muscle cells
UR - http://www.scopus.com/inward/record.url?scp=0034214332&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034214332&partnerID=8YFLogxK
U2 - 10.1006/jsre.2000.5891
DO - 10.1006/jsre.2000.5891
M3 - Article
C2 - 10816346
AN - SCOPUS:0034214332
VL - 91
SP - 32
EP - 37
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 1
ER -